Alpenglow Biosciences accelerates drug development and improves clinical diagnostics through AI-enabled 3D tissue imaging and digital pathology.
Seattle, WA
Joined December 2021
- Tweets564
- Following727
- Followers418
- Likes133
Pinned Tweet
🍽️ Dinner, drinks, and tumor microenvironment science at #SITC2026!
Join us on Nov. 5 in Phoenix for a complimentary dinner lecture with Stanford’s Jonathan T.C. Liu, PhD, exploring 3D pathology, molecular profiling, and tumor-immune interactions.
bit.ly/47yKymS
Alpenglow Biosciences ® retweeted
🍽️ Dinner, drinks, and tumor microenvironment science at #SITC2026!
Join us on Nov. 5 in Phoenix for a complimentary dinner lecture with Stanford’s Jonathan T.C. Liu, PhD, exploring 3D pathology, molecular profiling, and tumor-immune interactions.
bit.ly/47yKymS
Today, we're taking prostate glands for a spin! 🔬
This #FluorescenceFriday, explore a prostate biopsy in 3D, from H&E-like imaging to synthetic CK8 and gland segmentation.
📍 At #SITC2026? Join our Nov 5 dinner lecture!
alpenglowbiosciences.com/sit…
There's a lot more happening in fat than you might think! 🔬
This #FluorescenceFriday, we're revisiting a mouse fat pad in 3D, revealing branching blood vessels, nerve fibers, and macrophages throughout the tissue.
🔴 Lectin. Blood vessels
🔵 CD68. Macrophages
🟢 PGP9.5. Nerves
🍷 Heading to #SITC2026? Join us for dinner and science!
Professor Jonathan Liu will share his experience in 3D pathology, from guided microdissection to studying the tumor microenvironment.
📅 Nov 5 | 6–8 PM | Phoenix
Save your seat 👉 alpenglowbiosciences.com/sit…
Last week, the whole HS biopsy. This week, one stunning hair follicle! 🔬
High-resolution 3D imaging reveals the entire follicle, intricate nerve networks, and individual cells throughout the tissue depth.
From 3D to virtual slices and back. All measurable.
#FluorescenceFriday
📣 We’re bringing a new discussion to SITC 2026.
On November 5 in Phoenix, Jonathan Liu, PhD, will lead a dinner lecture on integrating 3D pathology with molecular and microenvironment profiling, followed by a panel discussion.
RSVP now:
alpenglowbiosciences.com/sit…
No AI here. Just the real Steve Pemberton, now on the ground in the UK. 🇬🇧
Cambridge → London → Oxford, talking light-sheet imaging, 3D spatial biology and quantitative analysis.
And with today’s Nobel putting light in the spotlight… Steve picked a pretty good week. 💡🔬
🔊 VOLUME UP. This HS biopsy comes with Strauss. 🎶
Travel through the tissue until you reach the HS tunnel. In 3D, you can follow its structure and surrounding nerves, follicles and mast cells, not just catch one 2D profile.
🧊 See enough of the tissue to trust the conclusion
Tomorrow at 1 PM EDT: join us for a closer look at rare tissue-resident memory T cells in vitiligo and what 3D tissue imaging can reveal beyond section-based analysis.
Featuring Cynthia Timmers, Incyte, and the Alpenglow team.
Register: us02web.zoom.us/meeting/regi…
Alpenglow is heading to the UK 🇬🇧
Oct 5–9, Steve and Andrew will be in Cambridge, London, and Oxford, meeting with research teams and imaging facilities to discuss 3D light-sheet imaging, instrument workflows, quantitative tissue analysis, and Summit AI™.
Write to meet with us!
Because “how many?” is only half the question.
The next one: where (for real)? 😏
🟢 B220+ B cells
🔴 CD3+ T cells
🔵 Nuclei
In an intact colorectal tumor, single cells stay mapped in full-volume context.
Numbers matter. Location gives them meaning.
#FluorescenceFriday
Alpenglow Biosciences ® retweeted
12/12 Incredible work led by the talented @Zliu1218 in Jeff Chuang Lab👏! Grateful to the team at @MoffittNews, @JAXcancercenter, @vizgen_inc, @alpenglowbio, @USouthFlorida, @DrJamesMule, @PatrickHwuMD, @VernSondakMD, @song_xiaofei, @MarkowitzLab, @JiangHe_PhD, @RederredeR
Alpenglow Biosciences ® retweeted
11/12
3D light-sheet imaging and CODA 3D reconstruction show germinal centers that look separate in 2D are often interconnected tunnels in 3D, raising real questions about whether single-section histopathology adequately captures neoadjuvant ICB response assessment.
Alpenglow Biosciences ® retweeted
10/12 We next leveraged this rare multimodal dataset, matched H&E, mIF, and IHC from real-world ICB-treated tumors, to train PathNet-TLS, an AI model that detects TLS/GCs directly from H&E and reproduces the same regimen-specific differences, a step toward clinical deployment.
Alpenglow Biosciences ® retweeted
9/12 GCSCAN enabled objective quantification of TLS/GC structures across our full cohort for the first time. GC/TLS density independently predicted disease-free survival, and notably, 75% of responders who later relapsed had low or absent germinal centers at surgery.
Alpenglow Biosciences ® retweeted
8/12 To solve this, we built GCSCAN, a graph-based algorithm that delineates TLS and GC structures from molecular data with single-cell precision, objective, reproducible, and generalizable across spatial omics and imaging platforms.
Alpenglow Biosciences ® retweeted
7/12 TLS and germinal centers show a striking association with neoadjuvant ICB response, yet current scoring relies on visual estimation of residual tumor and stroma, entirely missing TLS/GC as a distinct, quantifiable biomarker. Should this change?
Alpenglow Biosciences ® retweeted
6/12 SCIRA defined cell-cell communication in neoadjuvant ICB response, mapping receptor-ligand signaling across millions of cells at whole-slide scale. It revealed zone-specific circuits organizing the T cell, GC/B cell, and myeloid compartments of the NICB microenvironment.
Alpenglow Biosciences ® retweeted
5/12 To interrogate these high dimensional datasets, we developed 3 purpose-built computational tools: SCIRA for whole-slide single-cell receptor-ligand quantification, GCSCAN for molecularly grounded TLS/GC delineation, and PathNet-TLS for automated TLS/GC detection on H&E.