Light-regulated TurboID, or LOV-Turbo, is a feat of engineered allostery, created by @SongyiLee10 and @CheahJoleen via structure-guided design, then optimized by yeast display directed evolution. Activity is nearly undetectable in the dark state, but increases within seconds to a level comparable to TurboID after weak blue-light illumination. Removing light shuts LOV-Turbo off again (ie, it is reversible). LOV-Turbo is easy to use in mammalian cells, yeast, bacteria, and even in the mouse brain to suppress background labeling from endogenous biotin, to perform pulse-chase proteomics, and to spatially control biotinylation. We also show that instead of external blue light, LOV-Turbo can be activated by genetically-encoded light, ie bioluminescence from luciferase, via BRET (Figure 4F-I). Huge thanks to incredibly talented co-first authors @SongyiLee10 and @CheahJoleen, and our wonderful collaborators Steve Carr and @namude. Also many thanks to co-authors @Simenzhao, Charles Wu, @heegwangroh, @tinakim_neuro and @kelvinfcho for their contributions. Plasmids will be available on Addgene in the near future! biorxiv.org/cgi/content/shor…