Our mammalian cell programmable reaction-diffusion plasmid pack is now on Addgene--everything you need to get started making waves, analyzing images with DSP, and interfacing with cell biology! Have fun and reach out if you need help/have questions 😵‍💫addgene.org/browse/article/2…

Great thread highlighting some really nice natural and engineered examples of cooperativity (one of life's secret sauces) in TFs. Should do one for signaling scaffolds too...

And one last thank you to our reviewers for super helpful feedback & comments, our amazing editor, and the synthetic biology community which has been super supportive of this work over the last year. This is only the beginning of what we're up to in this space, so stay tuned!

Finally, plasmids for tinkering with this system will be on Addgene shortly. If you're excited about this technology or just enjoy psychedelia, grab these reagents and do a transfection--within 24 hours you'll be tripping. We're so excited to see where imagination will take you!

I'm so proud of the @UWMadison @uwmadisonipib @UWMadBiophysics @UWBiochem @UWBioMajor students that put this work together: first author @born2raisecell, @EdenChang_, and undergraduates @Tjmlg2012 and Elliott Weix are science superstars who weren't afraid to try out wild ideas.

Third: we've included a detailed "design manual" to help new users become familiar with reaction-diffusion systems, the MinDE components, and how they can be used to build and model more complex synthetic circuits. Length and timescales are super important to consider here!

Second: we added a new stability analysis to show that the frequency of an oscillation is quite consistent over the lifetime of a cell, even as it crawls around, changes shape or divides. We suspect drift in a cell's frequency may reflect underlying changes in its physiology...

First: this paper is bursting with new concepts and our amazing editor gave us the space and support to unpack and discuss these ideas fully. In particular, we've clarified the powerful frequency domain and time-frequency analysis image analysis tools, which really shine now!

Our first paper is out in @CellCellPress! Led by @born2raisecell, we present a programmable reaction-diffusion system that generates synthetic protein waves, oscillations, and patterns for spatiotemporal circuit design and FM data-encoding in human cells. authors.elsevier.com/a/1iN8Z…