@HelgeBode_Lab

Run by members of the Bode Lab. Doing all things related to natural products.

 Marburg, Hesse
mpi-marburg.mpg.de/natural-products-in-organismic-interactions
Joined March 2019
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  • Following55
  • Followers551
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Happy to announce the first steps into NRPS/PKS engineering thanks to the work from Leonard and collaborations with the labs from Markus Kaiser and Michael Groll: Bioengineering of syrbactin megasynthetases for immunoproteasome inhib... sciencedirect.com/science/ar…
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Leonard has applied the XUT NRPS engineering technology to solve the biosynthesis of the potent antibiotic odilorhabdin in collaboration with the Rolf Müller lab at @Helmholtz_HIPS. See his paper in @angew_chem: doi.org/10.1002/anie.2024063…

Investigation of the Odilorhabdin Biosynthetic Gene Cluster Using NRPS Engineering

The biosynthesis of the odilorhabdin biosynthetic gene cluster (BGC) was elucidated by engineering of the central non-ribosomal peptide synthetase (NRPS). Therefore, specific modules of the odilorh...

onlinelibrary.wiley.com
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CRISPR now also available for Xenorhabdus and Photorhabdus for easy access to natural products and manipulation of their biosynthesis: Please see the paper from Alexander in Microbial Cell factories: rdcu.be/dDibN
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And here is another cool story about trans-AT PKS engineering in the same issue science.org/doi/10.1126/scie… from the @piel_lab. Congratulations!!

Evolution-guided engineering of trans-acyltransferase polyketide synthases

Single-molecular tension sensors reveal cellular force loading rate during mechanotransduction.

science.org
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Finally! After a long journey our latest NRPS engineering story is out at science.org/doi/10.1126/scie… thanks to the hard work of several members from the @HelgeBode_lab

Evolution-inspired engineering of nonribosomal peptide synthetases

Bioinformatic analysis reveals sequence sites ideal for engineering in enzymes that produce non-ribosomal peptide natural products.

science.org
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With our newest preprint on engineering NRPS we succeeded in optimizing our first-of-its-kind biocombinatorial technology. Read more here: Guidelines for Optimizing Type S Non-Ribosomal Peptide Synthetases biorxiv.org/content/10.1101/…

Guidelines for Optimizing Type S Non-Ribosomal Peptide Synthetases

Bacterial biosynthetic assembly lines, such as non-ribosomal peptide synthetases (NRPS) and polyketide synthases, are often subject of synthetic biology – because they produce a variety of natural...

biorxiv.org
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The latest update for NRPS engineering, based on a evolutionary breakpoint within thiolation domains is out: doi.org/10.1101/2022.12.02.5…
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Next round in "the many things that regulatory RNAs can do" - this time: controlling natural-product synthesis. Fantastic collaboration with the Bode lab (@HelgeBode_Lab) published today in @NatureMicrobiol nature.com/articles/s41564-0…
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An awesome addition to polyketide biosynthesis. TE disguises itself as KS catalyzing branched polyketide formation. Read about Molecular Basis for the Biosynthesis of an Unusual Chain-Fused Polyketide Gregatin A pubs.acs.org/doi/10.1021/jac…

Molecular Basis for the Biosynthesis of an Unusual Chain-Fused Polyketide, Gregatin A

Abstract. Gregatin A (1) is a fungal polyketide featuring an alkylated furanone core, but the biosynthetic mechanism to furnish the intriguing molecular sk

pubs.acs.org
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Luckily, despite severe jet lag, Helge’s given a clear, nice talk @SIMB_Microbio #NaturalProducts on NRPS engineering with a more efficient approach. Showing the advantages of artificial docking domains in splitting and reconstructing the megasynthetases
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Finally... more than a module! Great NRPS work from the Schmeing lab: Structures of a dimodular nonribosomal peptide synthetase reveal conformational flexibility science.sciencemag.org/conte…
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Who makes antibiotics and how do NRPS work? See the Grininger/Bode group performance for kids here: rz.uni-frankfurt.de/68128012…
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🤔 could have submitted more
J Org Chem/Org Lett@JOC_OL12 Sep 2019
JOC invites you to take a look at this Note from first-time corresponding author Helge Bode & coworkers: (±)-Alternarlactones A and B, Two Antiparasitic Alternariol-like Dimers from the Fungus Alternaria alternata P1210 dx.doi.org/10.1021/acs.joc.9…
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Bode Lab retweeted
Piel Lab@piel_lab9 Sep 2019
We have new, hypermodified peptides for you! Check out our aeronamide (from Microvirgula) & polygeonamide (from deep-rock metagenome🤘🎸) story at @NatureChemistry nature.com/articles/s41557-0… #RiPPs
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Natural products go RNA: The Natural Product Butylcycloheptyl Prodiginine Binds Pre-miR-21, Inhibits Dicer-Mediated Processing of Pre-miR-21, and Blocks Cellular Proliferation cell.com/cell-chemical-biolo…
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Bode Lab retweeted
Piel Lab@piel_lab22 Jul 2019
Read Jörn‘s @nature #behindthepaper on how we developed the TransATor, a web tool for de novo structure predictions of trans-AT polyketides & why you need it go.nature.com/2jL18eL
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Bode Lab retweeted
Piel Lab@piel_lab15 Jul 2019
The long-awaited TransAtor paper is out in @nchembio! TransAtor is a web application enabling de novo structural predictions of trans-AT PKS-derived polyketides. nature.com/articles/s41589-0…
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First time to achieve peptide ligation in water, offering a chemical perspective of chicken-and-egg problem for the origin of life nature.com/articles/s41586-0…
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Nice work by the Stanley-Wall lab: Pulcherrimin formation controls growth arrest of the Bacillus subtilis biofilm pnas.org/content/116/27/1355…

Pulcherrimin formation controls growth arrest of the Bacillus subtilis biofilm | PNAS

Biofilm formation by Bacillus subtilis is a communal process that culminates in the formation of architecturally complex multicellular communities....

pnas.org
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Bode Lab retweeted
Piel Lab@piel_lab13 Jun 2019
Tom's amazing @NatRevChem review is finally out!! Learn more about hidden #enzymology, unprecedented chemistry & new approaches for exploring microbial dark matter at #naturalproducts nature.com/articles/s41570-0…
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