@IanYYen

Type IV Pilus | Aspiring e- cryo-microscopist | PhD Candidate | UofToronto | P. Lynne Howell lab 🇨🇦🔬🦠

lab.research.sickkids.ca/howell/
Joined March 2022
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Excited to share our new book chapter! We summarize recent advances in the structures and mechanisms of non-fimbrial adhesins in biofilm formation, with perspectives on upcoming discoveries in this field. #BiofilmResearch #Adhesins link.springer.com/chapter/10…

Structures of Fibrillar Adhesins That Mediate Bacteria–Host Inte

This chapter explores the structural and functional mechanisms of fibrillar adhesins involved in bacteria-host interactions and biofilm formation. Fibrillar adhesins are non-fimbrial proteins...

link.springer.com
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Just in time for #WAAW, collab with the Strynadka lab ⁦⁦@UBC -⁩ Cryo-EM characterization of the anydromuropeptide permease AmpG central to bacterial fitness and β-lactam antibiotic resistance. ⁦@McMasterIIDR⁩ ⁦@BBS_McMasterU⁩ ⁦#AMR nature.com/articles/s41467-0…

Cryo-EM characterization of the anydromuropeptide permease AmpG central to bacterial fitness and...

Nature Communications - AmpG is an MFS importer of bacterial cell wall fragments. Here, authors present a cryoEM structure and supporting mutagenesis to illuminate AmpG’s essential role in...

nature.com
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Our new review on regulation of #T4P type IV pilus assembly #buildingpermits, by PhD candidate Nathan Roberge, is now available for you to enjoy with your morning coffee! 😀 @McMasterIIDR @BBS_McMasterU @MacGlobalNexus @machealthsci journals.asm.org/doi/epub/10…
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Happy and proud to see this paper online! We resolved the in-situ architecture of the P. aeruginosa Type IV pilus machine and discovered the localisation and role of PilY1. Great collaboration with @JunLiuLab @Dr_Lori_Burrows @BrunLabCaulo @PLynneHowell . nature.com/articles/s41467-0…

PilY1 regulates the dynamic architecture of the type IV pilus machine in Pseudomonas aeruginosa

Nature Communications - Type IV pili (T4P) enable bacteria to sense, move, and adhere to surfaces. Here, the authors solve the structure of the T4P machine in Pseudomonas aeruginosa, revealing the...

nature.com
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Happy to share my first PhD paper from @PLynneHowell lab with my fantastic post-doc mentor @gregbwhitfield from @BrunLabCaulo as well as @Dr_Lori_Burrows and @RubinsteinJohn with #cryoem support where we propose how the bifunctional Tad pilus motor works🧵 biorxiv.org/content/10.1101/…
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14/ I would also like to acknowledge a complementary study from Harry Low's lab, where they propose a different CpaF catalytic mechanism. nature.com/articles/s41467-0…

Bidirectional pilus processing in the Tad pilus system motor CpaF

Nature Communications - The ATPase CpaF from C. crescentus powers pilus dynamics in the bacterial Tad pilus system. Cryo-EM and light microscopy characterisation of CpaF uncovers a mechanism for...

nature.com
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15/ Many thanks to members of my lab for their tremendous support and to @RubinsteinJohn lab members (@think_zesty, @CourbonGautier, @hanlinw222, Hui Guo, @JustinDiTrani, @SBenlekbir, Zhijie Li) for teaching me #cryoem from scratch. Happy reading! biorxiv.org/content/10.1101/…

Coordinated conformational changes in the Tad pilus ATPase CpaF facilitate a rotary mechanism of...

The type IV pilus family uses PilT-like ATPases to rapidly assemble and disassemble pilin subunits. Among them, the tight adherence (Tad) pilus performs both functions using a single bifunctional...

biorxiv.org
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1/ #CryoSPARC v4.6 is out! 🚀Up to 2.1x faster refinements and classification, a new job tree view that is scalable and performant on large #cryoEM projects, job groups, new cards and table view, automatic inspect picks, and more! cryosparc.com/updates
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