Importantly, enhancers lost after lncRNA depletion could be mapped with Hi-C to the promoters of differentially expressed genes. (6/7)

We next identified the top SWI/SNF-sensitive open chromatin regions using a SWI/SNF PROTAC and ATAC-seq. Enhancers 'lost' after PROTAC also corresponded to enhancers lost after lncRNA depletion. (5/7)

Remarkably, depletion of SWI/SNF-bound lncRNAs using an siRNA library followed by CUT&RUN revealed a LOSS of SWI/SNF binding at enhancers and a relative GAIN of binding at promoters. (4/7)

iCLIP revealed lncRNAs as the most positively enriched class of SWI/SNF-bound RNA. Binding of lncRNAs to SWI/SNF occurred at a higher frequency than would be expected. The top candidate lncRNAs were mostly trans-acting in nature, based on RedChIP data. (3/7)

SWI/SNF lacks a DNA recognition motif yet achieves precise enhancer targeting. Using RedChIP and CUT&RUN approaches, we show that RNA-DNA interactions are enriched with SWI/SNF. The majority of these RNAs are trans-acting and bind cell type-specific enhancers. (2/7)

Absolutely thrilled to have received the 2022 Rudi Busse award from the medical faculty of @goetheuni for my #PhD thesis on #lncRNA maintenance of #gene expression and #genome stability. Very nice ceremony tonight at the Dies Academicus in Frankfurt. – at Frankfurt on the Main, Germany

And even more delighted to have won an @RNASociety poster prize at #RNA23. Thanks again to everyone who came to my poster to have a chat and thanks to those who organised the meeting. @BrandesLab @trr267 @jrRNAscientists – at Singapore

Delighted to have been given the opportunity by the @RNASociety to present my work at #RNA23 #RNA2023 in #Singapore. Had great discussions about #lncRNA regulation of #chromatin complexes in #endothelial #gene #expression. @RNASociety @jrRNAscientists @BrandesLab – at Singapore

🚨Our paper on the role of #lncRNA PCAT19 in #endothelial quiescence, and its implications for #cardiovascular disease, has just been published in @CellReports! 🎉 Featured on cell.com/cell-reports/fullte…. #RNA #vascular @CellPressNews #DNA #ScienceTwitter – at Frankfurt on the Main, Germany

@BrandesLab #squad #goals #Europhysiology2022 – at Copenhagen, Denmark