Assistant Professor, Chemical Engineering Nanomaterials, Synthetic Biology, Protein Engineering, Biomaterials
Joined February 2022
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Can phase separation alone form a gel?
Our latest work shows that PEG–dextran phase separation can spontaneously produce viscoelastic gels without polymerization or chemical cross-linking. We also show these gels can encapsulate live cells, antibiotics, and plant seeds.
If you're interested in a new, minimally perturbative fluorescent labeling approach applicable to peptides, proteins, and enzymes, please take a look at our new contribution
pubs.acs.org/doi/10.1021/acs…
The answer appears to be yes.
Krishna and P. S. Sivaprasad led the protein engineering and experimental work, Reman Singh provided key simulation insights, and Saranraj Krishnan synthesized an important control peptide.
Silica nanoparticles are everywhere, but recovering them from water remains a challenge.
In our latest work, engineered supercharged GFP proteins act like molecular “bridges” to capture and aggregate silica nanoparticles entirely in water.
A fun example of proteins engineered beyond biology → sustainable nanoparticle recovery.
Read more: pubs.acs.org/doi/full/10.102…
@ACS_Langmuir
Our recent work, “Liquid–Liquid Phase Separation to Fabricate Microgels of Recombinantly Expressed Proteins,” is finally out!
Read the paper: lnkd.in/dA58Q9Jv
What makes this exciting is that it avoids emulsion-based or microfluidic methods, which often use oils or have limited throughput. Instead, it relies on intrinsic protein interactions — a gentle, biocompatible, and scalable route to microgel fabrication.
🔗
Our paper in Communications Biology (rdcu.be/eDDFZ) shows that a tiny 9-aa fragment from a light-cleaved protein doesn’t go dark — it aggregates and glows dim red.
Led by Yashwant Kumar & Krishna Agrawal. Grateful to @ANRFIndia for support!
Our latest from the lab — led entirely by students Aniruddha Kambekar, Vignesh Nandhagopal, Aditya Mehta & Yashwant Kumar (PhD)!
They built dual-layered hydrogels with spatially organized Au & Ag nanoparticles
Catalysis + photothermal in one.
🔗 pubs.acs.org/doi/full/10.102…
Karthik Subramaniam Pushpavanam retweeted
Our manuscript in @Biomaterials_
Histamine Receptor Agonism Differentially Induces Immune and Reparative Healing Responses in Biomaterial-facilitated Tissue Repair
#skinsealing
sciencedirect.com/science/ar…
@NIAMSFunding @NIBIBgov
@ASUBiodesign @ASUResearch @SEMTEatASU @jordanyaron
Are you passionate about protein engineering? We are looking for PhD candidates with an interest in molecular biology, protein engineering, and biotechnology.
Our research combines advanced molecular techniques to develop proteins with real-world applications in biosensing, environmental monitoring and therapeutic development.
We welcome all interested students, whether you have cleared DBT-JRF, CSIR-NET, or INSPIRE fellowships, or have a passion for protein design and related fields to discuss PhD opportunities in our lab (kpsubram[at]iitgn.ac.in).
This Teacher’s Day, my students surprised me with something truly special – they wrote about the regular phrases I use in our daily interactions. It’s incredible to see how something I say casually has become a (hopefully) lighthearted part of their day.
The production of recombinant proteins is central to biotech, yet downstream processing remains a major bottleneck. To tackle these issues, we’ve developed a light-activated molecular purification (LAMP) system using a photocleavable protein.
Explore LAMP pubs.acs.org/doi/10.1021/acs…
Traditional methods, like resin-based chromatography, face challenges such as nonspecific binding and complex elution buffers, which further enhance the final cost. This stimuli-responsive technique leverages light to purify proteins with precision.
Our studies show LAMP can achieve over 90% purity, offering a promising alternative to conventional methods. This work was spearheaded by Yashwant Kumar, Krishna Agrawal and Manisha Ojha. We would like to thank @gsbtm for their funding support.