An absolute pleasure to host our annual Research Facilities Open Day last month! Some inspiring talks from staff and users, fab sponsor stands, and great attendance from many new faces 🧑🔬💚 Read this article to find out more about how it went! @FbsResearch biologicalsciences.leeds.ac.…
University of Leeds PPF retweeted
Research facilities open day.
Good to go. We're excited to talk about our technologies and hear facility user stories.
Thank you Faye and Ruth for organising. Thank you sponsors for support.
@ScienceLeeds
Second in the Research Facilities to be awarded their gold LEAF award a few months ago! Great work @BioimagingLeeds 🥇✅
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AKTA cleaning day in the Protein Production Facility today 🫧
Have you remembered to do your system CIP this summer?
dH2O ✅
0.5M NaOH✅
dH2O✅
20% EtOH✅
#cleanAKTA #AKTAmaintenance #FacilityMaintenance #CleanScience
Day one of the Yorkshire Sustainability Conference.
Some great talks so far from Pam Warhurst and Jo Fairley on changing the face of food, and Areeba Hamid and Clover Hogan ‘from protest to progress’.
#yorkshiresustainabilityweek
Be curious! Come and see if you can find the virus infected cells on the microscope at the virology stand at @BeCuriousLeeds today 🦠
Thanks to @BioimagingLeeds for lending the microscope 🔬
@ScienceLeeds @UniLeedsEngage #BeCurious #Virology #ScienceIsFun
UoL users, come along to our drop-in sessions for any advice and to share results 👇
Events supporting users of our Biomolecular Interactions & Protein Production facilities.
Biacore SPR, Refeyn mass photometry, Fidabio hydrodynamics & protein purification.
More info at the Facilities Sharepoint site.
Apologies, UoL access for these.
@LeedsPPF @FbsResearch
Thank you for all of your suggestions - there were some really great ones!
Here are our recommendations...
We should have the result next week 🤞 so keep an eye out.
/Faye & Iain
Replying to @LeedsPPF
LOTS of protein but lots of cells & some proteolysis.
Change 1 thing - use 1-2L. Shorter time to resuspend, lyse, load & wash. Change another thing – check wash [imidazole]
That yield allows a classic CIPP – capture, intermediate & polish purification. Affinity, IEX, SEC.
How good are your protein purification skills?
What would you recommend for the facility user with this result? We’ll post our recommendations later.
SDS-PAGE of His-tagged protein from a 5ml Ni-NTA column. Cells from 6l of E. coli culture.
/Faye
University of Leeds PPF retweeted
For UoL facility users - join us on Teams for these 👇 drop-in discussions. More info on the Facilities Sharepoint.
SPR, ITC and Refeyn assays.
Making better reagents using protein expression & purification.
First Gold LEAF accreditation awarded to PIXC lab! sustainability.leeds.ac.uk/n…
University of Leeds PPF retweeted
Finally- cells!
Sally standing in for green fingered Fiona on plant growth
Tom dropped the 🎤(rograph) on #teamtomo + CLEM
James blasting us into the gas phase with proteomics
Sally brightened our day on fluorescence microscopy and flow
Brian bringing the digital to PCR 💪
University of Leeds PPF retweeted
Next up it's structures-
Chi with a highly ordered talk on X-ray crystallography!
Josh @JBR_White on the coolest technique in town - single particle cryoEM!
Arnout with a magnetic presentation on bioNMR!
James weighing in structural mass spec techniques!
University of Leeds PPF retweeted
Replying to @FbsResearch
@FbsResearch intro to facilities day- morning is molecules..
Brian's talk had no cell-by date as he talked about protein production!
James gave a masspectacular talk!
Iain had us captivated about interactions!
No polarising views from Nasir's talk on Circular Dichroism!
🤣
University of Leeds PPF retweeted
Fantastic talk by the @COMICResearchUK team 👏 #LYPFTResearch22
Next up is the COMIC team. Their research isn’t published yet so I can’t tweet it. Looks like the male domination of research is changing…#LYPFTResearch22
Great opportunity for a #PhDposition in Leeds School of Design, working with the Leeds protein production lab and wider FBS research facilities to investigate producing recombinant sustainable textile fibres
@FbsResearch #Sustainability #PhDStudent
phd.leeds.ac.uk/project/1237…
University of Leeds PPF retweeted
Wu lab is recruiting a 3-year postdoc, please RT!
This post =DNA damage response🧬 + cryoEM 🔬+ we support you and your career development ♥️
Job Opportunity at University of Leeds: Research Fellow in Structural Biology jobs.leeds.ac.uk/FBSMB1219
Great opportunity to join our Research Facility community, supporting exciting research, and working in the friendly and supportive Protein Production Lab
jobs.leeds.ac.uk/FBSMB1216
@ScienceLeeds @AstburyCentre @FbsResearch #technician #Leeds #ScienceJobs




























