Immune cell dynamics, migration, and diversity in cells & people @mcgillu. Account run by an exceptional team of trainee-clowns 🧑🔬🤡, with critical PI input.
Montréal, Québec
Joined June 2022
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Check out our recent publication in collaboration with the Khadra Lab at @mcgillu - where computational modelling 🖥️ and experimental immunology 🔬join forces to study the role of T cell diversity and antigen binding strength on chronic infection clearance! Find out more 👇
>90% of #TCR sequence diversity is generated by TdT-mediated nucleotide addition, but what is the benefit of TdT-altered TCRs? @MandlLab show that low-affinity #Tcells generated by TdT are important for curtailing chronic pathogen replication #PLOSBiology plos.io/3HJKOCl
Our PhD student Angela @ImmuneVet co-host of the TWiV podcast with @profvrr recently interviewed @bjesseshapiro and Corinne Maurice with their students Sana Naderi and Anshul Sinha about their cool virus research going on at @mcgillu! @McGillMed 📺bit.ly/3Hb9Aet
And this work featured on the current issue cover! Amazing collaboration with @JohannesTextor @MelicharLab, and the Westermann team
Wondering what we can learn from the T cell receptor beta sequence about the self-reactivity of a T cell? check out our paper in Cell Systems where we used machine learning to ask this very question👇: cell.com/cell-systems/fullte…
So proud! 🎉🌈
Find out more about this research from Connie Shen @peepingcells in our ‘First person’ interview:
journals.biologists.com/jcs/…
ALT Connie Shen
ALT A frame from a confocal microscopy time-lapse featuring differentially stained neutrophils migrating through a microfluidic device.
We add to the growing appreciation of neutrophils as a heterogenous population by characterizing differences in nuclear lobulation with respect to migratory behaviour 🌈
Our study provides evidence supporting the theory that nuclear segmentation facilitates neutrophil navigation of tight spaces, assisting their role as first-responders 🚒👨🚒
In our second approach, we isolate all the neutrophils and annotate the number of lobes in each. We track these neutrophils over time and also observe that greater lobularity correlated with greater cell speed and displacement across the channels.
Across donors, we find that the segmented and hypersegmented neutrophils more readily cross the restrictive 2 μm section compared to their banded counterparts.
With @Vrisekoop team at @UMCUtrecht, we devised a system to leverage the heterogeneity in neutrophil nuclei arising from the administration of endotoxin in human donors. Neutrophils released into circulation in this model have nuclei ranging from banded to hypersegmented.
Why do neutrophils have multi-lobed nuclei? 🧐
Does this enable their rapid migration through tight spaces? 🏃♀️💨
In this study, we sought to answer this question experimentally 🔬
Here is what we found 👇
Connie Shen @peepingcells, @MandlLab @mcgillu, @Vrisekoop @UMCUtrecht and colleagues discover that nuclear segmentation allows neutrophils to migrate faster in confined spaces.
Highlight: journals.biologists.com/jcs/…
Report: journals.biologists.com/jcs/…
#OpenAccess #ReadandPublish
Practicing our Night Science (@ItaiYanai) in our first Mandl lab retreat with @DanielaQuail @loganawalsh labs at the Gault Reserve
So excited to be chairing a session on Mechanosensing in Immunity with Sue Tsai @SueYTsai1 at the upcoming 35th Canadian Society for Immunology conference: csi-sci.ca/2023_Programme.ht… featuring Spencer Freeman @spencerSK1, Morgan Huse @huselab, Pablo Vargas, and Valerie Weaver.
Thank you so much @CdnImmunol for the shout-out 😍! It's such a privilege to be highlighted along so many other talented scientists during Women's History Month. Here's to another year of women-led science 🥳👩🔬
Dr. Judith Mandl @MandlLab is an associate professor at McGill University and is a CRC in Immune Cell Dynamics. Her lab investigates how immune cell migration, cell-cell interactions, and naïve T cell heterogeneity orchestrate the tightly choreographed dance of an immune response
Our very own Angela @ImmuneVet PhD student in the Mandl lab is a guest on TWIV this week! check it out ⬇️🦇💩 podcasts.apple.com/ca/podcas…
Connie & Jeremy enjoying #celllavie2 sbcf.fr/en/event/cell-la-vie… in Paris showing off our newest movies of human neutrophils and T cell crowds.
First PhD student from the Mandl lab went off to @MIT @ChoiLaboratory and we are still looking for her in our lab meetings. We can't wait to see what she does next!
