Graduate Student, Kampmann Lab, UCSF From Phoenixville, Pennsylvania Microglia and ice cream enthusiast
San Francisco
Joined April 2021
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I am excited to share our work in which we conducted a CRISPRi-based screen to assess 102 Autism Spectrum Disorder (ASD) risk genes in microglial uptake of synaptic material! (1/7)
biorxiv.org/content/10.1101/…
Olivia Teter retweeted
CRISPR has the potential to cure many genetic diseases, but it’s notoriously hard to use in neurons.
In this work we tackled 2 of the main hurdles: Cas9 delivery and DNA repair.
And we discovered…
The rules of genome editing are different in neurons👇🏾
biorxiv.org/content/10.1101/…
Microglia activation is commonly seen in ASD and abnormal microglial synaptic remodeling has been speculated to contribute to ASD biology. While expression of ASD risk genes is enriched in neurons, we were surprised to see that microglia express many ASD risk genes! (2/7)
We dove deeply into characterizing how ADNP affects microglia. We found loss of ADNP leads to decreased endocytosis, a remodeled surface proteome, and increased motility. (6/7)
Lots of gratitude and awe for everyone who helped contribute to this work through mentorship, scientific advice, and collaboration! @MartinUCSF, @DraegerNina, @sydsat, @AmandaKMcQuade, Brandon Holmes, and @KampmannLab(7/7)
UCSF's Institute for Neurodegenerative Diseases is hosting an open house on November 15, 4-6pm for interested rotation students. Come hear flash talks from each lab and afterward socialize with lab members! See you there!
Registration: forms.gle/inFDHPeRwbAWNacu6
Yes! In addition to upholding transparency and truly promoting scientific dialogue, this new publishing landscape will stand as a great training resource - reading peer reviews will surely help trainees, like myself, think critically about their own research
Today, we’re introducing a new model that eliminates accept/reject decisions.
By publishing every paper with eLife reviews as a Reviewed Preprint, we plan to restore autonomy to authors, ensuring that they will be judged by what, not where, they publish. elifesciences.org/inside-eli…
Amazing - this work reflects exactly why @DraegerNina inspires me; it’s fearless, thoughtful, and bright. Cheers to you, @sydsat, the whole @KampmannLab and @LiGanLab teams, and microglia, of course!🎉
It’s out! Our @NatureNeuro paper introducing an iPSC-Microglia protocol and screening platform that identifies modulators of microglia functions and states: “A CRISPRi/a platform in iPSC-derived microglia uncovers regulators of disease states.” nature.com/articles/s41593-0…
🧵(1/15)
Leaving beautiful Colorado with new friends, fresh ideas, and unparalleled excitement to study molecular mechanisms of synaptic pruning. Thank you organizers and amazing scientists at #KSNeuroImmune22!
