@OpenCellCZB

OpenCell is a collaborative proteome-scale project to measure human protein localizations and interactions led by the @LeonettiManuel lab @czbiohub

 San Francisco, CA
opencell.czbiohub.org
Joined March 2021
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Our paper is now out in @ScienceMagazine! We endogenously tagged 1,310 human proteins and systematically measured their localization and interactions. Check out opencell.czbiohub.org to explore our images and interaction networks! science.org/doi/10.1126/scie…
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One of the super useful features of the opencell.czbiohub.org website is that you can look up the cellular copy numbers and concentrations of your favorite proteins - check it out! (even for proteins that aren't among our targets or their interactors)
Manuel Leonetti@LeonettiManuel11 Mar 2022
Our paper is out today in @ScienceMagazine, I’m stoked @OpenCellCZB ! Check out opencell.czbiohub.org: CRISPR tagging + 3D live imaging + IP mass spec to map the human proteome. science.org/doi/10.1126/scie… 👇 tweetorial from our 2021 pre-print.
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OpenCellCZB retweeted
elevyLab@ElevyLab15 Mar 2022
Check our perspective on the @OpenCellCZB awesome work from @LeonettiManuel "Modules" help interpret biological systems. However, we see in these new data that connectors far outweigh modules in PPI networks! What does that mean? We discuss it: science.org/doi/10.1126/scie…
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All of our data is freely available to explore and download at opencell.czbiohub.org/downlo…. Special thanks to the AWS Open Data program for hosting our collection of 6,000 raw 3D microscopy images: czb-opencell.s3.amazonaws.co…
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This is just the beginning! We are continuing to tag new proteins and we are working on a way to distribute our existing library of cell lines. Stay tuned for new releases of our web portal and datasets!
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Our paper is out today in @ScienceMagazine, I’m stoked @OpenCellCZB ! Check out opencell.czbiohub.org: CRISPR tagging + 3D live imaging + IP mass spec to map the human proteome. science.org/doi/10.1126/scie… 👇 tweetorial from our 2021 pre-print.
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Published today in Science, a team from CZ Biohub and @MPI_Biochem combined genome engineering, confocal live-cell imaging, mass spectrometry, and data science to systematically map the localization and interactions of human proteins. 🧵 science.org/doi/10.1126/scie…

OpenCell: Endogenous tagging for the cartography of human cellular organization

Combining genome engineering, live-cell imaging, mass spectrometry, and data science are used to map the localization and interactions of human proteins.

science.org
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.#OpenCell from @czbiohub -- results from which are published in @ScienceMagazine today -- is mapping cells one protein at a time, paving a new way to visualize cellular architecture. science.org/doi/full/10.1126…. Video: youtube.com/watch?v=6j4fpV-q…
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Excited to see this update! Cytoself enabled us to quantitatively compare our IP/MS interaction data with our microscopy images (spoiler alert: we showed that if proteins in our library share similar localization patterns, they are more likely than not to directly interact!)
Hirofumi Kobayashi@liilii_tweet9 Jul 2021
We have an updated preprint of cytoself, our deep-learning method for profiling protein localization with self-supervised learning! Fantastic collaboration with @kchev, @LeonettiManuel and @loicaroyer. (1/8) biorxiv.org/content/10.1101/…
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Cellular life is amazingly complex. To find cures for diseases, we must understand the molecular details of how cells and their proteins operate. Visit our new OpenCell website and interactively explore the localization and interactions of human proteins:opencell.czbiohub.org
Manuel Leonetti@LeonettiManuel30 Mar 2021
Endogenous tagging + 3D live-cell imaging + mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB
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Our first release is live! 🚀 A collaborative effort led by @LeonettiManuel with @labs_mann and @loicaroyer and many others at @czbiohub to build an open-source map of the human proteome. Check it out and let us know what you think! opencell.czbiohub.org
Manuel Leonetti@LeonettiManuel30 Mar 2021
Endogenous tagging + 3D live-cell imaging + mass-spec interactome = opencell.czbiohub.org, an open-source map of the human proteome. So excited for our first release, wonderful collaboration with @labs_mann @loicaroyer & many other colleagues. @OpenCellCZB
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OpenCell: proteome-scale endogenous tagging enables the cartography of human cellular organization biorxiv.org/cgi/content/shor… #bioRxiv
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Self-Supervised Deep-Learning Encodes High-Resolution Features of Protein Subcellular Localization biorxiv.org/cgi/content/shor… #bioRxiv
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Our first release is live! 🚀 A collaborative effort led by @LeonettiManuel with @labs_mann and @loicaroyer and many others at @czbiohub to build an open-source map of the human proteome. Check it out and let us know what you think! opencell.czbiohub.org
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