@RivelineLab

Research Team in Biological Physics

 Strasbourg, France
riveline.igbmc.science
Joined June 2020
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xitter.cf/Cellphysics/status/166…
Cellphysics Master@Cellphysics5 Jun 2023
New Science Sketches from the Cell Physics Master students sciencesketches.org sciencesketches.org/student-… @SciSketch @unistra
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A fruitful interaction with @Honigmann_Lab in our @HFSP network with Masaki Sano and @anne_grapin Labs. xitter.cf/MarkusMukenhirn/status…
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Jan. 2023 Call : The Cell Physics Master recruits now for September 2023 with international lecturers and practicals in Strasbourg (France) : cellphysics-master.com Applications cellphysics-master.com/conta… 3D imaging of an eye of a live zebrafish N. Peyriéras and A. Klymchenko
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In a separate study using experiments & continuum model, we show that cell stiffness dependent height variations are important for generating the pulsations in monolayers & in setting their lengthscales (doi.org/10.1140/epje/s10189-…). 7/7
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We used a vertex model to understand the transition to pulsations & flows. We found that a dominating velocity-polarity alignment led to flows but when the velocity-polarity alignment matched the rotational diffusion, pulsations emerged. 6/7
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Thus by tuning external substrate friction & the internal cytoskeletal organization we can force the monolayers to behave in a unique way - pulsate/flow. We also found correlated myosin accumulation & lamellipodial alignment during pulsations & flows, respectively. 5/7
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As a standard, we then checked the role of cytoskeleton using inhibitors. The inhibitors suppressed the pulsations, but when we tried to get back the pulsations by washing out & restoring cytoskeletal activity, there were no pulsations – but long range flows. 4/7
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Using spatially periodic micropatterns of various sizes, we trap the pulsations & demonstrate that substrate friction regulates pulsation sizes. By increasing the relative friction between adhesive patterns & PEG regions, we further substantiate the role of friction. 3/7
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We observed the dynamics of large confluent MDCK monolayers & found spontaneously emerging, locally oscillating & flowing domains (10-15 cells ~ 200 µm). Characterizing them using divergence & velocity magnitude, we asked what determines their lengthscales. 2/7
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Our study from @Raghavan_Thiaga came out recently in @iScience_CP (doi.org/10.1016/j.isci.2022.……). We show how two coexisting collective epithelial dynamics - pulsations & flows - are regulated. 1/7
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Another Movie showing Cell motility in 3D Matrix. Our article with @PierreSens and collaborators appeared in @eLife. #CellMotility #CellMigration @unistra @INSB_CNRS @INP_CNRS @IGBMC doi.org/10.7554/eLife.71032… @eLife
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Our review on computational approaches to study luminogenesis with @HiraiwaTetsuya and co-authors just appeared : sciencedirect.com/science/ar…
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Replying to @HiraiwaTetsuya
Thank you to Tetsuya Hiraiwa and all co-authors for our review on computational approaches to study luminogenesis.
#FRANCE: The Cell Physics Lab. @unistra offers support for a scientist at any stage through a Human Frontier Science Program special call. The research involves cell biology, microscopy, micro-fabrication, theory. scienceforukraine.netlify.ap… #ScienceForUkraine
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We celebrated today 4 recent PhDs at @DevStem_Igbmc who graduated from the first years of the new @Cellphysics Master @unistra : Basile from @CharvinLab, Simon from @RivelineLab, Gui from @molina_lab, Hélène from @julien_vermot Congratulations to each of them.
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New call for short stays from students and researchers @unistra in Dresden, sponsored by the city hall of Strasbourg @strasbourg in the twin city program Strasbourg-Dresden @stadt_dresden strasbourg.eu/dresde-allemag…
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The side view of a migrating NIH 3T3 cell with the same setup as Movie S8. The video can now be dowloaded from the comment section : doi.org/10.1016/j.bpj.2014.0…
Another perspective : The side view of a migrating cell - live. For more details, see our article #Comelles et al. Biophys J. 107:1513–1522 (2014). doi.org/10.1016/j.bpj.2014.0…
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Epithelial colonies in vitro elongate through collective effects. Our article with @JordiComelles and Mandar Inamdar just appeared in @eLife elifesciences.org/articles/5… @unistra @INSB_CNRS @INP_CNRS @IGBMC in collaboration with #mpipks @iitbombay @TheCrick
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Ratchetaxis in channels: entry point and local asymmetry set cell directions in confinement. Our #ratchetaxis channel article just appeared in @BiophysJ cell.com/biophysj/fulltext/S… #CellMotility #CellMigration @unistra @INSB_CNRS @INP_CNRS @IGBMC
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