@SamResnick

Columbia MD/PhD Student

 NY, NY
Joined December 2010
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A multiplex platform to identify mechanisms and modulators of proteotoxicity in neurodegeneration dlvr.it/SYdsMB
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My second paper from grad school! We worked with Peter St George-Hyslop, Gabriele Kaminski Schierle (@mng_ceb), and the @RuggeriLab A multiplex platform to identify mechanisms and modulators of proteotoxicity in neurodegeneration biorxiv.org/content/10.1101/…

A multiplex platform to identify mechanisms and modulators of proteotoxicity in neurodegeneration

Neurodegenerative disorders are a family of diseases that remain poorly treated despite their growing global health burden. A shared feature of many neurodegenerative disorders is the accumulation of...

biorxiv.org
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-We identified DNAJB6 as a chaperone with activity against multiple ALS/FTD associated models including TDP-43, FUS, and hnRNPA1 in both yeast and mammalian cell culture -We showed with multiple in vitro techniques that DNAJB6 modulates FUS's propensity to aggregate
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-We also did some deep mutational scanning on DNAJB6 and identified variants with enhanced activity! -Really couldn't have done this without our collaborators! This project has been on my mind daily for many many years and I'm very pleased to be able to share it
My first publication from grad school! In collaboration with the David Ho and Brent Stockwell labs, we developed a simple cell culture-based assay to identify protease inhibitors targeted at SARS-CoV-2 and other coronaviruses #COVID19 biorxiv.org/content/10.1101/…

A simplified cell-based assay to identify coronavirus 3CL protease inhibitors

We describe a mammalian cell-based assay capable of identifying coronavirus 3CL protease (3CLpro) inhibitors without requiring the use of live virus. By enabling the facile testing of compounds...

biorxiv.org
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The assay we developed works well for numerous coronavirus proteases and many known protease inhibitors. We get potency data highly similar to data produced from live virus assays. We can identify compounds that are broadly active against strains with the ability to infect humans
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If your lab has 293T cells, crystal violet, and a plate reader, you can do this assay! It's reporter-free, transfection-based, and robust to protocol variation. We hope this assay will expedite therapeutic identification and will be highly useful in the case of future pandemics
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Our newest manuscript is live on bioRxiv! Beautiful work led by Leo @Vopinator in the lab, for high-efficiency, multiplexed DNA insertions in bacteria using CRISPR RNA-guided integrases. A great collaboration w/ @RondaCarlotta and @harriswangnyc. 1/n biorxiv.org/content/10.1101/…

CRISPR RNA-guided integrases for high-efficiency and multiplexed bacterial genome engineering

Tn 7 -like transposons are pervasive mobile genetic elements in bacteria that mobilize using heteromeric transposase complexes comprising distinct targeting modules. We recently described a Tn 7...

biorxiv.org
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Sam Resnick retweeted
Businessweek
@BW19 Mar 2020
⚡️The world's most famous AIDS researcher is racing to find a coronavirus cure xitter.cf/i/events/1240641677204…
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A First-of-its-Kind Library of Potential Coronavirus Drugs | Time time.com/5790709/coronavirus…
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. @FitBiomics, a startup formed by @TrueHoopsNY, @geochurch and @adkostic from the @wyssinstitute, is set to develop highly validated #probiotics based on elite athletes’ #microbiomes, to improve athletic performance and conceivably, overall health. bit.ly/2LvMlhM

Harvard’s athlete-based next-generation probiotics technology licensed to FitBiomics

By Benjamin Boettner (BOSTON) — Today the Wyss Institute for Biologically Inspired Engineering at Harvard University announced that its athlete microbiome-based technology will be commercialized by...

wyss.harvard.edu
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2,304 PCRs today... thankful for multichannels. Anyone else out there do an absurd amount of the same thing in one day?
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Sam Resnick retweeted
ShorterLab@ShorterLab29 Jul 2019
RPS25 is required for efficient RAN translation of C9orf72 and other neurodegenerative disease-associated nucleotide repeats, great work from ADG, LP, @isaacs_adrian et al: nature.com/articles/s41593-0…
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Thrilled to post this preprint, the result of a really fun collaboration with @IsraelF96135088 led by the very talented Tyler @HalpinHealy! Our first glimpses into the machinery that directs RNA-guided DNA insertion, and how CRISPR and transposition proteins work together!
Tyler S Halpin-Healy@HalpinHealy18 Jul 2019
Hot off the press from the @SternbergLab biorxiv.org/content/10.1101/… Can't thank @SanneKlompe @shsternberg @IsraelF96135088 enough for superhuman effort.
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Great paper by @TrueHoopsNY @FitBiomics
Eric Topol
@EricTopol24 Jun 2019
Whoa. The gut #microbiome of elite athletes (marathon runners) has a bacterium that enhances performance, makes mice run longer, and this is mediated via lactate metabolism @NatureMedicine nature.com/articles/s41591-0… (file under more #microbiome amazing stuff)
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