@Stjle

Synthetic Biology, Artificial Cells, Amphiphile Systems, Machine Learning, Cell-Free Protein Synthesis: Italy, Denmark, Japan, USA, France, Germany, UK.

 Rome, Lazio
scholar.google.co.uk/citations?user=u6OhA2oAAAAJ&hl=en
Joined April 2010
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25Media
Pleased to share my latest review on Cell-Free Protein Synthesis #CFPS and #AI/ML for accelerating drug discovery sciencedirect.com/science/ar… #CellFree #SynBio
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Filippo Caschera retweeted
Olivier Borkowski@O_Borkowski6h
Decoding & manipulating human intercellular signaling via scalable cell-free protein synthesis doi.org/10.64898/2026.10.24.…
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Development of isopentenol utilization pathway based on cell-free metabolic engineering and its application in multienzyme cascades synthesis sciencedirect.com/science/ar…
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通信系(IEEE)分野にやや風変わりなperspectiveを書きました(せっかくなので最後に「パケットの進化」とか好き放題言っている Cell-Free Systems and Nucleic Acid Packets for Robust Molecular Communication ieeexplore.ieee.org/abstract…

Cell-Free Systems and Nucleic Acid Packets for Robust Molecular Communication

Cell-free systems provide an experimentally tractable basis for molecular communication because they decouple communication chemistry from the physiological variability of living cells and enable...

ieeexplore.ieee.org
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Rapid Production and Cryo-EM Structure Determination of GPCR-Nanodisc Complexes via Cell-Free Synthesis biorxiv.org/content/10.64898…

Rapid Production and Cryo-EM Structure Determination of GPCR-Nanodisc Complexes via Cell-Free...

G protein-coupled receptors (GPCRs) represent major therapeutic targets, yet cryo-EM structure determination of unliganded or antagonist-bound GPCRs remains severely bottlenecked by small receptor...

biorxiv.org
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Discovery of a Novel High-Salt-Adapted Archaeal AP Endonuclease using Microfluidics-Based Cell-Free Functional Metagenomics biorxiv.org/content/10.64898…

Discovery of a Novel High-Salt-Adapted Archaeal AP Endonuclease using Microfluidics-Based Cell-Free...

We report the discovery of a novel archaeal AP endonuclease recovered from a metagenomic library constructed from environmental DNA collected in a salt-marsh ecosystem. Using an ultra-high-throughp...

biorxiv.org
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A semi-automated cell-free protein synthesis workflow for quantifying antibiotic-inactivating enzymes sciencedirect.com/science/ch…
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Filippo Caschera retweeted
shimizu@musaibouOct 15
On chip 50S assembly pubs.acs.org/jacsat/article/…

Autonomous Biogenesis of Early and Late Assembly Intermediates of a Large Ribosomal Subunit in a...

Abstract. Cell-free autonomous biogenesis of ribosomes is important for the bottom-up assembly of an artificial cell and for engineering of functional ribo

pubs.acs.org
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Photo-Activatable RNA Switches for Optical Control of Cell-Free Protein Synthesis pubs.acs.org/asbcd6/article/…

Photo-Activatable RNA Switches for Optical Control of Cell-Free Protein Synthesis

Abstract. Programmable RNA switches enable target-dependent regulation of cell-free protein synthesis. Here, we report a photo-activatable RNA switch using

pubs.acs.org
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Modular Cell-Free Extract Mixing Enables Soluble, Active Biomanufacturing of Difficult-to-Express and Aggregation-Prone Proteins biorxiv.org/content/10.64898…

Modular Cell-Free Extract Mixing Enables Soluble, Active Biomanufacturing of Difficult-to-Express...

Cell-free protein synthesis (CFPS) is a powerful platform for rapid protein prototyping and biomanufacturing, but expression of complex proteins remains limited by labor-intensive extract preparation...

biorxiv.org
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Gene-driven self-morphing microtubule-based active matte nature.com/articles/s41563-0…

Gene-driven self-morphing microtubule-based active matter

Nature Materials - Cell-free gene expression is embedded directly within a microtubule network to generate a genetically programmed active material with complementary biochemical and physical...

nature.com
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Cells, who needs them? Biochemists turn to proteins in a tube nature.com/articles/d41586-0…

Cells, who needs them? Biochemists turn to proteins in a tube

Nature - Scientists are using cell-free transcription and translation systems to synthesize proteins, design biology-based sensors and develop synthetic cells.

nature.com
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Filippo Caschera retweeted
Riku Nagai@riku_nagai_eduOct 3
Congrats to everyone involved! frontiersin.org/journals/mol…

Frontiers | Full-protein release assay in a reconstituted yeast translation system using GGQ-meth...

Translation termination in eukaryotes requires the concerted action of release factors eRF1 and eRF3, together with accessory factors including eIF5A and Rli...

frontiersin.org
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiOct 2
Mitigating the deleterious impact of saliva on cell-free expression sensors for point-of-use diagnostics doi.org/10.1093/synbio/ysag0…
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A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that binds the peptidyl transferase center nature.com/articles/s42003-0…

A human cell-free translation screen identifies the NT-2 mycotoxin as a ribosomal inhibitor that...

Communications Biology - A human cell-free translation screen identifies the fungal toxin NT-2 as a ribosomal inhibitor that binds the peptidyl transferase center and is associated with...

nature.com
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiSep 25
Cell-free Engineering of LPST153 Bacteriophage Receptor-Binding Proteins Enables Tunable Host-Range Modulation doi.org/10.1021/acssynbio.6c…
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Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange circuits for programmable protein expression biorxiv.org/content/10.1101/…

Cell-free systems enable mechanistic characterization of genetically encoded RNA strand exchange...

Nucleic acid circuits are powerful tools for programming biology, but the design principles for operating these circuits in complex cellular environments remain poorly understood compared to simple...

biorxiv.org
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Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-associated CaCA2 variants biorxiv.org/content/10.64898…

Cell-free membrane systems reveal electrostatic control of ion transport in TMEM165 and disease-a...

Ion transport across intracellular membranes is essential for cellular homeostasis, yet organellar transport mechanisms remain difficult to resolve in native cells. Here, we establish a cell-free...

biorxiv.org
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Cell-free systems as complementary Test layers for protein engineering in biofoundry workflows sciencedirect.com/science/ar…
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Filippo Caschera retweeted
Olivier Borkowski@O_BorkowskiSep 15
Engineered Orthogonal Translation Systems from Metagenomic Libraries Expand the Genetic Code doi.org/10.1021/acssynbio.6c…
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visualize.jove.com/42729265-…

A liquid handling platform for standardized quantification of cell-free enzymatic activity encoded...

Rapidly characterize antimicrobial resistance genes (ARGs) with a new semi-automated workflow. Essential for combating AMR and developing new antibiotics.

visualize.jove.com
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