(he/him) Light Microscopist. Tweets about microscopy 🔬, cell biology, and optics. No new posts here, but find me on bluesky: @tanner-fadero.bsky.social
Redwood City, CA
Joined August 2017
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Tanner Fadero retweeted
We present long-term stabilization of an oblique plane microscope (OPM).
By stabilizing the remote focus unit of an OPM, we can enable long-term acquisition without light-sheet drift.
The feedback signal comes from a laser, no fluorescence is expended.
biorxiv.org/content/10.1101/…
Tanner Fadero retweeted
Correction from @TanFad! It's actually the The Center for Advanced Light Microscopy (CALM) @UCSF that is building the #Snouty microscope! They have a great team there with @nico_stuurman, @KariHerrington and @micaelalasser (Instagram). A great location!😀
Tanner Fadero retweeted
PSA: Heat-set inserts make stronger threads in a 3D print, and look quite tidy.
Tanner Fadero retweeted
Following a poke from @RunningPhoton, I've fixed a bug in my KL divergence calculation function which means our parameter-free deconvolution approach now *really* has no hand-tuned parameters at all:
beryl.mrc-lmb.cam.ac.uk/rlgc…
Feedback always welcome.
xitter.cf/JamesDManton/status/18…
We (@AndrewGYork, @RunningPhoton, @microRussell and I) are still working on the manuscript describing our automatic parameter-free deconvolution approach, but we've made a Google Colab notebook where you can try it out without needing to install anything:
beryl.mrc-lmb.cam.ac.uk/rlgc…
Tanner Fadero retweeted
Did you know that nuclear pore complexes can turn up outside the nuclear envelope? Studying these, @JennySachweh, Bernhard Hampoelz and others uncover that the surrounding cellular milieu determines NPC composition via the nuclear transport system itself. #teamtomo #NPC A 🧵
Tanner Fadero retweeted
Where is the optical axis? Finding it can be critical to building microscopes. Here's the back reflections at ~15cm from a collimated ~1mm⌀ 532nm laser aligned into the back of Nikon 40x0.95 air objective. Radially symmetric/concentric interference rings indicate good alignment.
Tanner Fadero retweeted
Thermal expansion -> a typical problem in time-lapse microscopy at high resolution. Here I measure the axial shift of the focal plane of a Nikon 40x0.95 air objective at ~1.6um/K, or about ~1.5x the depth of field per degree C:
github.com/amsikking/Nikon_4…
Tanner Fadero retweeted
Long awaited (thanks @JamesDManton for posting the patent application!) and now out in @ScienceMagazine: Using absorption to look deeper by @HongNeuroTech - they achieved approx. 3 mm depth using the food dye tartrazine: #Microscopy
science.org/doi/10.1126/scie…
Tanner Fadero retweeted
This study, assessed as fundamental by reviewers, identifies protein kinases in the parasitic protozoan, #Toxoplasma gondii involved in host cell invasion drug resistance. elifesciences.org/articles/9…
Tanner Fadero retweeted
I've now made a few small changes to our parameter-free deconvolution code to better handle low SNR data, and to add MIPs to the notebook so you have a feeling for the results without needing to download images from Google Drive.
xitter.cf/JamesDManton/status/18…
We (@AndrewGYork, @RunningPhoton, @microRussell and I) are still working on the manuscript describing our automatic parameter-free deconvolution approach, but we've made a Google Colab notebook where you can try it out without needing to install anything:
beryl.mrc-lmb.cam.ac.uk/rlgc…
Tanner Fadero retweeted
One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology.
Norman RX, Chen YC, Recchia EE, Loi J, Rosemarie Q, Lesko SL, Patel S, Sherer N, Takaku M, Burkard ME, Suzuki A.
doi.org/10.1101/2024.08.13.6…
Tanner Fadero retweeted
Pier Andrée Penttilä (@MRC_LMB Flow Cytometry facility), Rose Morag Hunter (@AstraZeneca) and I are looking for a postdoctoral scientist to develop a multispectral light-sheet imaging flow cytometer and apply this to phenotypic cell sorting: nature.com/naturecareers/job…
Tanner Fadero retweeted
Sure matplotlib is cool, but what if I want to load my loss curves into the 2006 hit Flash game LineRider?
Tanner Fadero retweeted
We (@AndrewGYork and I) are still working on the manuscript describing our artefact-free, noise-appropriate, resampling approach, but we've made a Google Colab notebook where you can try it out:
beryl.mrc-lmb.cam.ac.uk/upsc…
Below, we go from 104 nm per px to 8 nm per px.
Tanner Fadero retweeted
Nice @MBLScience article featuring myself+@GladfelterLab #MBLPhysiology and others:
mbl.edu/news/mbl-course-dire…
Tanner Fadero retweeted
Exciting job opportunity! 🚀
My team at @czbiohub SF is hiring a motivated and ambitious Research Assistant! (Bachelor or Master level) Join us to work at the intersection of developmental biology🥚, cutting-edge imaging🔬, and molecular biology & genomics🧬. You will learn a lot™. Apply now! :
job-boards.greenhouse.io/cha…
#molecular_bio #lightsheet #imaging #zebrafish #czbiohubsf
Tanner Fadero retweeted
We discovered a new species: Stentor stipatus! We named it for its brown aggregates💩 We explore its morphology, phylogeny, and behavior in my new preprint with Daniel Cortes and @WallaceUcsf. This single cell exhibits habituation and phototaxis! bit.ly/4fwSasv
🧵1/n
Tanner Fadero retweeted
A while ago we embarked on the most exciting project with @gomez_mariscal at @HenriquesLab. Phototoxicity Fitness Time Trial or #PhotoFiTT assay is a label-free method to measure cellular behavior and detect phototoxic effects during live-cell imaging biorxiv.org/content/10.1101/…
Tanner Fadero retweeted
🚨🔔🚨 We have another postdoc opening in our group to study lipid trafficking in neurons. If you have a background in mechanistic cell biology and lipids (lipidomics= bonus!) and are interested in employing your skills in neurons- Apply! Link below - closes 22nd July. please RT!
Tanner Fadero retweeted
We have integrated adaptive optics in an oblique plane microscope, where one deformable mirror corrects both the light-sheet and the fluorescence detection.
opg.optica.org/boe/fulltext.…
Figure: cancer cells in a zebrafish xenograft model, before and after application of AO. 1/4
