physician-scientist studying fundamental mechanisms of biology in infectious amoeba. https://teapawt.org/t.co/ojEXqellAQ
Madison, WI, USA
Joined January 2013
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Our paper "Mitochondrial proteome of Acanthamoeba delineates aerobic and anaerobic pathways dynamically regulated by oxygen" is online now @CellCellPress! cell.com/cell/fulltext/S0092…. Grateful to my amazing post-doc mentor @VamsiMootha and all the other team members!
Jonathan Stefely, MD, PhD retweeted
A @NobelPrize for a Badger 🏅
Congratulations to Francis Halzen, named the 2026 Nobel laureate in physics for his groundbreaking work with @uw_icecube to advance our understanding of neutrinos and the universe’s most energetic events.
news.wisc.edu/university-of-…
Jonathan Stefely, MD, PhD retweeted
Remarkable Magnum Opus from @VamsiMootha & the Mitocarta Tree of Life consortium consisting of 9 back to back research papers (+2 perspectives) just released in Cell and Cell family journals:
- Characterization of mito proteomes from 6 diverse species, from Giardia to Leishmania to Arabidopsis
- Optimized methods for mito enrichment, DIA mass spec, protein correlation profiling, microscopy validation, and cryoEM analysis of native complexes
- Comparative analysis reveals dozens of proteins shared among pathogens but lost in human hosts = “pan-parasite” drug targets
- Data used to re-train ML predictor of subcellular localization => predicted mitoproteomes across hundreds of eukaryotes
- Giardia solute carrier discovered with functional validation
- Dynamic rewiring of Acanthamoeba mito proteome from aerobic to anaerobic energy production characterized
And tons more… The papers are instant landmarks and raise even more questions than they answer, paving the way for many future investigations of this fascinating organelle #mitochondria
cell.com/consortium/mitocart…
Jonathan Stefely, MD, PhD retweeted
Proteomics Maps Eye-Infecting Acanthamoeba Mitochondria Across Oxygen Levels
The Acanthamoeba mitoproteome was mapped, revealing oxygen-driven metabolic shifts and potential targets for safer infection treatments
@jastefely @VamsiMootha
#proteomics
hubs.li/Q04z2r5z0
Jonathan Stefely, MD, PhD retweeted
Our new issue features the MitoCarta Tree of Life (MitoTOL) Consortium👉cell.com/cell/current
On the cover: a transmission electron micrograph of mitochondria , digitally rendered in the style of a watercolor painting.
Jonathan Stefely, MD, PhD retweeted
I (fondly) remember as a TA insisting Berkeley undergrads memorize the Krebs cycle and OxPhos ('it's good for your soul,' id say). We'd draw the Krebs cycle as a loop floating free in the mitochondrion, and the respiratory chain as a separate machine in the membrane. In one organism they are bolted together.@VamsiMootha
doi.org/10.1016/j.cell.2026.…
Jonathan Stefely, MD, PhD retweeted
Proud to share the fruits of a 4 year effort with an amazing team at the MitoCarta Tree of Life Consortium! Today, we report insights on the origin and evolution of mitochondrial proteomes across billions of years: cell.com/consortium/mitocart…
mitocarta.org
The @Morgridge_Inst for Research is hiring a new metabolism-focused Investigator! Joint tenure-track at UW-Madison, outstanding colleagues, support, facilities, and resources! Apply to join us in Madison! morgridge.org/job-posting/me…
Our paper on a new genome annotation for the pathogen Acanthamoeba castellanii is now online at @cellpressblue! cell.com/cell-press-blue/ful…. This was a key foundation for our separate AcMitoCarta paper. Grateful to John Samuelson and @VamsiMootha for their mentorship!
Jonathan Stefely, MD, PhD retweeted
Today, the MitoCarta Tree of Life Consortium (MitoTOL) is releasing high-accuracy mitochondrial proteomes across diverse eukaryotes to empower studies of physiology, evolution, and disease:
cell.com/consortium/mitocart…
All data can be accessed at:
mitocarta.org
Jonathan Stefely, MD, PhD retweeted
In a 28-year-old woman with postpartum immune thrombotic thrombocytopenic purpura, plasma exchange was unsuccessful. Near death while receiving vasopressors, she was treated with recombinant ADAMTS13, and the disease abated. Read the Brief Report: nej.md/4b77CsZ
ALT Brief Report Recombinant ADAMTS13 for Immune Thrombotic Thrombocytopenic Purpura A peripheral smear obtained on day 0 (50× magnification) after application of a computerized algorithm for the automated detection of schistocytes (blue shaded cells).
Recombinant ADAMTS13 for Immune Thrombotic Thrombocytopenic Purpura | New England Journal of Medicine nejm.org/doi/full/10.1056/NE… @MGHPathology Blood Transfusion Service
Jonathan Stefely, MD, PhD retweeted
Out today @Nature – our analysis of the influences of common nuclear DNA variation on mitochondrial DNA copy number and heteroplasmy across >250,000 people across two biobanks! disq.us/t/4ibyjjo
Blood clots can develop in patients with #COVID19, but the mechanisms are unclear. Could we predict patients at high risk? We started to learn more about this while working on the @MGHPathology coagulation service during the pandemic peak: massgeneral.org/news/press-r… @MassGeneralNews
Jonathan Stefely, MD, PhD retweeted
Dr. @katrinarmstrong, Chief of @MGHMedicine at @MassGeneralNews, expertly discusses the differential dx for this week's @NEJM #CaseRecords:
Case 16-2020: 48W w/ Recurrent #Melanoma & Pulm Nodules.
Congrats Drs. Armstrong, @DrJustineCohen, Shepard, Folch, Mansour, @jastefely!
Jonathan Stefely, MD, PhD retweeted
After 10+ fantastic years in Madison, it is bittersweet to announce that my lab and I are moving to @WUSTLmed soon. I am so grateful to everyone at @UWBiochem and @Morgridge_Inst for their support and guidance. Stay healthy, and come visit us in St. Louis!
medicine.wustl.edu/news/pagl…
Jonathan Stefely, MD, PhD retweeted
Very proud of lab alumna Danielle Lohman, an amazing biochemist now applying her skills to US science policy. Check out this @cenmag article about Danielle and other recent PhDs now engaged in #scipol. cen.acs.org/content/cen/arti…
Jonathan Stefely, MD, PhD retweeted
One of our PGY1 CP-resident, Dr. @jastefely, presenting at @NEJM Clinicopathological Conference 🔬
Phenomenal #NEJMCPC by #Doctor Katrina Armstrong, @MGHMedicine Chair and @MassGeneral Physician-in-Chief!
47W
#Immunotherapy for recurrent #melanoma
Returned from #SouthAmerica
New pulmonary nodules on @MGHImaging
Eval by @MGHCancerCenter and @mgh_id
Diagnosis by @MGHPathology
