@leepaowan

PhD student - EPFL LBNC

 Lausanne
Joined March 2021
  • Tweets14
  • Following267
  • Followers52
Hey synthetic biology folks! We’ve developed a new T7 RNAP regulatory toolbox to help you build gene circuits! It includes methods to design programmable repressors, activators, and biomolecule sensors — ready to integrate into your T7 RNAP-based cell-free systems.
Maerkl Lab@MaerklLab19 Jun 2025
Pao-Wan, Saeed and Matthis' paper is out now on BioRxiv: "A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications" biorxiv.org/content/10.1101/…
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A synthetic cell with integrated DNA self-replication and membrane biosynthesis biorxiv.org/cgi/content/shor… #biorxiv_synbio
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A neural network, made out of proteins and built in mammalian cells. "we combined de novo–designed protein heterodimers and engineered viral proteases to implement a synthetic protein circuit that performs winner-take-all neural network classification..." Congrats @ElowitzLab.
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Excited to share my first publication with the Maerkl Lab! 🎉 Our review offers a comprehensive overview of the mechanisms and applications of various regulatory methods. If you’re looking to construct gene regulatory networks or need components to start with, check it out!
Maerkl Lab@MaerklLab8 Nov 2024
Pao-Wan's review on cell-free regulatory components is out now! Regulatory Components for Bacterial Cell-Free Systems Engineering | ACS Synthetic Biology pubs.acs.org/doi/10.1021/acs…

Regulatory Components for Bacterial Cell-Free Systems Engineering

Abstract. Cell-free systems are advancing synthetic biology through fast prototyping and modularity. Complex regulatory networks can now be implemented in

pubs.acs.org
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Pao-Wan Lee retweeted
Maerkl Lab@MaerklLab8 Dec 2022
Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with Hydrogel Membranes for Continuous Small Molecule Dialysis Barbora and Laura's paper is out now. pubs.acs.org/doi/10.1021/acs…

Improved Cell-Free Transcription–Translation Reactions in Microfluidic Chemostats Augmented with...

Abstract. Increasing the protein production capacity of the PURE cell-free transcription–translation (TX-TL) system will be key to implementing complex syn

pubs.acs.org
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Getting ready for my talk tomorrow at @UUtah @janetiwasa, featuring my beloved whole-cell model of course 🤩
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Scientists uncover the answer to a long-standing question in biochemistry: How do fatty-acid binding proteins deliver fatty acids to membranes? Read the @jbiolchem paper. ow.ly/gU0K50HOCoJ
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Pao-Wan Lee retweeted
Sáenz Lab@LabSaenz4 Feb 2022
Small reunion/and farewell to celebrate former PhD student @SandraRaouf starting her first job at @BiotypeDx and @leepaowan getting accepted at @EPFL_EDBB to start his PhD! Great way to end the week!! 🎉🎊🎈🍻
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Scientists from the @UofIllinois @chemistryuiuc, JCVI, and @tudresden_de have developed a computer model of the minimized synthetic cell, JCVI-syn3A, that accurately predicts the growth and molecular structure of its real-life counterpart. jcvi.org/media-center/scient…

Scientists develop most complete whole-cell computer simulation model of cell to date

LA JOLLA, CA—January 20, 2022—Scientists from the University of Illinois at Urbana-Champaign, J. Craig Venter Institute, and Technische...

jcvi.org
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Pao-Wan Lee retweeted
Sáenz Lab@LabSaenz19 Jan 2022
Our latest manuscript "Lipid membranes modulate the activity of RNA through sequence-dependent interactions" is... 🎉🎊 published 🎊🎉!! pnas.org/content/119/4/e2119… @t_czer_jea @James_Saenz @PNASNews @VolkswagenSt @BMBF_Bund @SimonsFdn @BCUBE_TUDresden
Sáenz Lab@LabSaenz31 Mar 2021
Lipids and RNA have something in common: they are essential for life, and most people don’t want to work on them. Now we show that everyone’s least favorite biomolecules actually interact in a useful way. How? biorxiv.org/content/10.1101/… @t_czer_jea @James_Saenz
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