Excited to share our latest study! Today in @naturemethods the @schwanklab and @krauthammerlab report in @marquark et al. 'Effective genome editing with an enhanced ISDra2 TnpB system and deep learning-predicted ωRNAs' (1/6) nature.com/articles/s41592-0…

Happy to share that my postdoctoral work is now out in @ScienceMagazine. We show that the RNA-programmable bridge recombinase ISCro4 can insert, delete, or invert multi-kilobase DNA fragments at defined genomic sites in human cells. Study link: science.org/doi/10.1126/scie… CRISPR-Cas has transformed genome editing, but many diseases involve diverse patient-specific mutations within the same gene. A mutation-agnostic alternative is to insert a healthy gene copy at a defined genomic locus, but gene-sized, site-specific insertions remain a major challenge. Main findings of our work: (1) ISCro4 is highly active in human cells and can be delivered by plasmid or all-RNA formats (2) Proof-of-concept programmable multi-kb insertions, deletions, and inversions (3) Structural insights into the basis of enhanced ISCro4 activity (4) Specificity and off-target characterisation (5) A framework to support future development and adoption of bridge recombinases The work was made possible through a close collaboration between the Jinek Lab, @schwanklab, and @jcornlab. I am deeply grateful to all of my co-authors for the team spirit, hard work, and dedication that went into this publication: @talasandris, Javier Fernández Carrera, @nicopmat, Lilly van de Venn, Charles Yeh, @p_kulcsar, @marquark, @YanikWeber, @SaskiaGerecke, Isabelle Harvey-Seutcheu, Dominic Mailänder, @MorPfl, and @ChrisChanez89. Special thanks to my supervisor, Prof. Martin Jinek, for his outstanding mentorship, and to Prof. Gerald Schwank and Prof. Jacob Corn for their generous support throughout. Finally, I would like to thank everyone in the Jinek lab for creating a supportive work environment, and @EMBO for funding my postdoctoral work. In parallel, independent work led by @ntperry13, @SKonermann and @pdhsu also reported ISCro4 activity in human cells, reinforcing the robustness and momentum of this direction. Please reach out if you would like to test the system or discuss potential applications. Relevant plasmids are now available via @Addgene.

🚀 AI meets CRISPR: a new era of genome editing is here. We’re excited to announce the publication of our review, “Harnessing artificial intelligence to advance CRISPR-based genome editing technologies” in @NatureRevGenet ! In this work, we and many thought-leaders explore how AI-driven methods—from deep learning to language models—are powering the next generation of genome editing: optimizing guide RNAs, engineering novel enzymes, and ultimately accelerating therapeutics and functional biology. 🔬 Highlights: --AI-powered design and prediction tools for CRISPR/Cas systems (nuclease, base, prime editing) ---Virtual cell/organ models to guide target selection and outcome prediction ---Roadmap for integrating AI into editing pipelines—from tool discovery to clinical translation 📘 Full paper → nature.com/articles/s41576-0… Proud to contribute to this intersection of computational biology, machine learning, and gene therapy — the future of precision medicine is being built today. Shoutout to all the co-authors for their valuable contributions! Particularly my friend and colleague @BowenLi_Lab for his leadership! @UHN @UofT

Excited to share our new protocol in #NatureProtocols! 🧬👨‍💻🎉 A comprehensive guide for using PRIDICT2.0 and ePRIDICT machine learning tools to design efficient #CRISPR-Cas9 prime editing experiments. 🧵 @UZH_Science @schwanklab @krauthammerlab nature.com/articles/s41596-0…

Our paper describing an RNA-LNP prime editing strategy for treating phenylketonuria was published today in @natBME (1/7) nature.com/articles/s41551-0… @schwanklab @UZH_Science @UZH_en @KispiZuerich @ETH_en

Seven early-career researchers were awarded UZH Entrepreneur Fellowships this year. The jury was impressed by their innovative product ideas and motivation to bring them to market as entrepreneurs: news.uzh.ch/en/articles/news… @marquark @UZH_IPT @CelestinMUTUYI1 @PardisAdams @UZH_Chemistry