@mattszarz

PhD student at UC Berkeley || Plant and Microbial Biology🌿 🧫

Joined May 2015
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Matthew Szarzanowicz retweeted
Ben Williams@WilliamsSciMar 31
We have been cooking up this story for a while and we are excited to finally be able to share! Read on if you're interested in whole plant regeneration WITHOUT the application of hormones!
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Matthew Szarzanowicz retweeted
Nick Desnoyer
@NickDesnoyer12 Dec 2024
In one year, I genetically engineered the boring Arabidopsis into a beautiful ornamental flower 🧬🌹 Here is how and why I gave this model organism a visual upgrade 🧵(1/7)
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I am pleased to announce new research from the Shih Lab (@LabShih) published today in Nature Biotechnology on improving Agrobacterium-mediated transformation via binary vector copy number engineering (doi.org/10.1038/s41587-024-0…). 🧵A summary thread below🌿

Binary vector copy number engineering improves Agrobacterium-mediated transformation

Nature Biotechnology - Agrobacterium-mediated transformation of plants and fungi is enhanced by plasmid copy number variants.

nature.com
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More validation of these mutants (including with an additional construct) can be found in the SI data of the paper (along with this beautiful Ruby Arabidopsis gradient!).
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We hope to now focus our attention on characterizing the transformation outcome and determine the relationship between binary vector copy number, transformation efficiency, and stable insertion number in diverse systems. Plenty of fun science to come!🧑‍🔬🌿
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Matthew Szarzanowicz retweeted
Mark Budde 🦕🏆
@markwbudde25 Jan 2022
Primordium is now offering everyone the chance to sample our full-plasmid sequencing service for FREE - never use Sanger sequencing again! Please retweet this message and we'll DM you a coupon to sequence 5 ENTIRE PLASMIDS 100% FREE, no CC or PO needed. primordiumlabs.com 5/
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No roots yet but a nice red flower! Must be quite stressed in there, but I’m quite pleased with how it matured over the holiday! @ATinyGreenCell
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Still can’t get over just how beautiful betalain-producing tissues are! Shown is tobacco (N. benthamiana) callus expressing the #Ruby construct for betalain biosynthesis🌱🔴 (1/2) #plantbiotechnology #gmo #transgenic #biotech
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The first of hopefully many cute little red meristems!❤️
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Fun with chromoproteins! Depicted is E. coli expressing a gene from the coral Acropora millepora🌊 This gene is useful in cloning by serving as an indicator for empty vectors. Swapping the chromoprotein for another gene will produce a white colony—easy positive colony ID!🧫(1/2)
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The original gene I received produced a blue protein. By modifying just 2 amino acids, the vibrant pink was produced. Small changes in a protein’s primary structure can have pronounced effects on the final product—wonderful biological plasticity! #biotechnology #microbiology
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