Postdoc in AbuGoot lab @omarabudayyeh @jgooten Formerly @Lab_Nagy at @LBI_RUD / @CeMM_News
Cambridge, MA
Joined August 2020
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So excited to share our new paper out today in @Nature. We repurposed R2 retrotransposons to create STITCHR, a tool that can make diverse edits in mammalian genomes including scarless multi-kilobase scale insertions
nature.com/articles/s41586-0…
Chris Fell retweeted
Our paper on the cryo-EM structures of Bxb1 has finally been published in Molecular Cell after a two-year journey through Science and Molecular Cell! Thanks to a great collaboration with @ChrisWFell, @omarabudayyeh, and @jgooten.
sciencedirect.com/science/ar…
🚨 Today we report cryo-EM structures of Bxb1 integrase complexed with its attB/attP DNA substrates before, during and after recombination.
We engineer Bxb1 mutants with improved recombination and altered sequence recognition properties.
Out today @MolecularCell ⬇️
This was the result of a great collaboration with @hnisimasu lab!
Many thanks to all authors! @somturn20 @_Dariomics_ @BenjaminAryaMD @jgooten @omarabudayyeh and all others not on twitter
Chris Fell retweeted
I discuss the urgent crisis from the loss of federal support of science in the US and recent clinical gene editing breakthroughs with @WalterIsaacson & @amanpour on @AmanpourCoPBS, airing on @PBS tonight at 11 pm ET, and on @cnni earlier today. Pls share!
youtube.com/watch?v=8YhJM6zx…
Chris Fell retweeted
I've been waiting for the day I could make this video!
Retrotransposons are reprogrammed for pretty efficient gene insertion up to 12.7kb in user-defined genomic targets. Small deletion + insertion possible too.
Awesome work from @jgooten @omarabudayyeh @ChrisWFell @lukas_villiger in @Nature
Chris Fell retweeted
In @nature we describe the use of scalable #proteinengineering & #machinelearning to predict millions of bespoke CRISPR-Cas9 enzymes, offering safer & more genome editing tools. 🧬🖥️
@RachelSilvers11 @CGM_MGH @MGH_RI @MassGeneralNews @harvardmed
nature.com/articles/s41586-0…
Chris Fell retweeted
In a new study, researchers from @MassGenBrigham and colleagues showed that a new gene editing tool could help treat disease with multiple mutations. The study was published in @Nature.
Read more: massgeneralbrigham.org/en/ab… @jgooten @omarabudayyeh
Chris Fell retweeted
🧬 New in Nature: STITCHR is a programmable, scarless RNA-templated integration system
It fuses site-specific retrotransposons to Cas9 nickase to install payloads from 1 bp to 12.7 kb - no DSBs, no scarring.
Let’s break down what it is and how it compares to Tessera, $PRME, and $BEAM
[1/]
Chris Fell retweeted
Happy to announce that our paper on the cryo-EM structures of CRISPR-Cas13 and REPAIR has been published in NSMB! Congratulations, Junichiro!
nature.com/articles/s41594-0…
Plasmids are now available from @Addgene !
So excited to share our new paper out today in @Nature. We repurposed R2 retrotransposons to create STITCHR, a tool that can make diverse edits in mammalian genomes including scarless multi-kilobase scale insertions
nature.com/articles/s41586-0…
This was a really fun project which I’m excited is finally out. There were some unique challenges on the way, including learning how to Southern blot to address a reviewer’s concern. The blot got buried in the supplemental, but it took me months, so here it is in all its glory
Lastly, I’m very thankful of my excellent co-first authors @lukas_villiger, Justin Lim, Masa Hiraizumi, and mentors @omarabudayyeh @jgooten. We are so grateful for our fantastic ongoing collaborations with @hnisimasu. Thanks to all co-authors for their invaluable contributions!