ALT Omics research in low- and middle-income countries (LMICs) is crucial for addressing global health issues but faces unique challenges. LMICs bear a significant disease burden, and omics technologies can help understand disease mechanisms and develop targeted interventions. However, adoption lags due to resource constraints. Barriers include administrative, institutional, project-specific, and community challenges, such as bureaucratic hurdles, lack of infrastructure and trained personnel, funding issues, and cultural obstacles. Strategies to overcome these barriers include streamlining processes, building local capacity, ensuring adequate funding, and engaging communities. Investing in omics research in LMICs is essential for identifying unique disease determinants, adapting interventions, and stratifying populations based on health needs. Addressing these challenges can significantly impact global health outcomes in LMICs. © Photo by Dr. Trenton Dailey-Chwalibóg