Find me at https://xitter.cf/t.co/HHs0sDXjO6 PhD @mpi_mr_hd (Johnsson lab) @HBIGS_HD Labeling strategies for super-resolution microscopy
Heidelberg
Joined April 2018
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Interested in the exchangeable HaloTag Ligands (= HaloX) technology for STED, PAINT and MINFLUX?🔬
Join the webinar hosted by @Abberior on September 21st and meet me online to discuss your questions afterward!
Are you tired of watching your fluorescent HaloTag® labels fade away during crucial live-cell imaging sessions?
We have the perfect solution for you!
Join our Webinar👇
🌟September 21, 2023
🕒15:30 (CEST)
🌐 register-HaloX.abberior.com
#abberior #imaging #halotag #HaloX #livecellimaging #LIVERED #STED #Confocal #livecell #GoLive #webinar
HaloX is licensed by @spirochrome
Julian Kompa retweeted
🎊🎉Exciting news: @RomanSarott will join us in January as a new Research Group Leader and will establish the group “Chemically Induced Proximity”. We look forward to seeing you again in January, dear Roman! 🤩👏mr.mpg.de/14599628/chemicall…
Julian Kompa retweeted
Bye, bye bleaching – hello #abberior LIVE HaloX!
HaloX is an exchangeable label that transiently binds to #HaloTag fusion proteins, allowing long-term protein detection during live-cell imaging. Say goodbye to bleaching issues and capture extended imaging periods with ease! Read more: abberior.rocks/dyes-labels/a…
HaloX licensed by @spirochrome
#abberior #STED #LiveCellImaging #halo #halotag #confocal #imaging #superresolutionmicroscopy #microscopy #fluorescentmicroscopy #nanoscopy #superresolutionSTEDmicroscopy
Exchangeable #HaloTag Ligands unlocking a new imaging modality. Another great collaboration (as always) with @HeilemannLab ! 😊
Long-term single-protein tracking up to 30 min allowed monitoring the dynamics of EGFR activation in living cells (and more)👇🏻Great work by a fantastic team and great collaboration with @JulianKompa Kai Johnsson @mpi_mr_hd 🙏 @sfb1507 @goetheuni
Julian Kompa retweeted
New paper on the role of mDia-family formins in cell cortex function of adherent cells out in @ScienceAdvances !
Huge thanks to Jan 'Hans' Faix for some of the best #STED #Superresolution samples I’ve seen to date. It was pleasure to contribute here.
science.org/doi/10.1126/scia…
ALT NIH 3T3 cell labeled with Phalloidin-STAR635P and recorded by STED super-resolution microscopy.
Julian Kompa retweeted
A big thank you to everyone involved: Lennart Nickel,
@hsi_yin, @JulienHiblot and Kai Johnsson.
For more background on the split-HaloTag system, check out our original paper here:
science.org/doi/10.1126/scie…
Gentle Rhodamines offer reduced phototoxicity for live-cell imaging of DNA, actin, mitochondria, the plasma membrane and (exchangeable) #HaloTag! 🔬
pubs.acs.org/doi/10.1021/acs…
Thanks @ZhixingChen2 and @TianyanL for this fruitful collaboration 😊
Our Gentle Rhodamines is finally published @ACSCentSci.
See the healthy cytoskeleton free from photodamage even after 800 frames! 🦾💪
pubs.acs.org/doi/10.1021/acs…
Great collaboration with Julian Kompa, the Johnsson group, and Spirochrome! @JulianKompa @spirochrome @TianyanL
Julian Kompa retweeted
What is Matter to Life? 🎬 We made a movie to answer this and to capture our science, community and beyond! To @mpi_mr_hd @UniHeidelberg @uniGoettingen @DKFZ @mpi_nat @mpids, and especially our students participating in the shoot - THANK YOU!
bit.ly/3XrWxND
Julian Kompa retweeted
🚨Excited to share my latest postdoc work in this #biorxiv preprint on developing split-HaloTag recorders for capturing cellular transient kinase activity, Kinprola (kinase activity dependent protein labeling).
biorxiv.org/content/10.1101/… 🧵 (1/7)
Julian Kompa retweeted
very impressed by the iSIM. We could acquire a whole volume of a gene edited cell (endogenous expression level so pretty dim!) in just a second. This is ARF1-mStayGold 🤩🤩🤩🤩 look at all those compartments!
#SMLMS2024 in Lisbon was incredible!
Great discussions in one of the most beautiful cities I've ever seen. Thanks to the organizers for a smooth conference and for awarding me with the 2nd poster prize ☺️🥈
Last week, #SMLMS2024 started! Considering we had an earthquake hitting Portugal just days before, we can say it was definitely groundbreaking. Hope you all had an amazing meeting!
Looking forward to seeing you again at Bonn's #SMLMS2025 hosted by @UEndesfelder and @HeilemannLab
Tired of your SNAPtag labeling underperforming in live cell imaging experiments? @SteffiKuehn has fixed that for you with the new SNAP-tag2 and the corresponding substrates 💡
Preprint alert🚨Did you ever experience issues in labeling kinetics and fluorescence brightness, when working with SNAP-tag in live cells?
Our newly developed SNAP-tag2 system might be the solution!
🚀Check out our latest preprint on #bioRxiv: 🔗biorxiv.org/content/10.1101/…
(1/4)
Thanks for having me as a teacher, just 2 years after I attended the course myself! 🙂
I really enjoyed discussing with the participants, having a glimpse on the new Leica TauSTED Xtend and showcasing 2-color imaging with exchangeable HaloTag Ligands 🔬
#EMBLSuperResolution just flew by! Last week, our participants learned how to design experiments using STED microscopy, prepare high-quality microscopy samples, and acquire state-of-the-art nanoscopy images. 🔬
We want to say thank you to everyone who joined, we hope you can take your new knowledge and use it back in your home institutions!
A big thank you also goes out to our co-organisers @LeicaMicro
Julian Kompa retweeted
Friedel-Crafts type reaction takes place with a base, instead of Lewis acid??? I am excited to share with you my first publication on sulfoamide synthesis during postdoc stage with Prof. Kai Johnsson! @mpi_mr_hd #MyFirstChemSci pubs.rsc.org/en/content/arti…
Under red-light illumination, silicon rhodamine undergoes photo-conversion to... 🥁 rhodamine! I'm glad someone looked into this; might be another step towards understanding the bleaching process of popular rhodamine / #HaloTag labels. 😊
Red Light Mediated Photo-Conversion of Silicon Rhodamines to Oxygen Rhodamines for Single-Molecule Microscopy biorxiv.org/cgi/content/shor… #bioRxiv
Julian Kompa retweeted
the lab is recruiting scientists @HHMIJanelia! we are particularly excited about engineering fluorescent proteins and biosensors using high-throughput assays and machine learning. questions? please get in touch!
Julian Kompa retweeted
Join abberior and your fellow superresolution microscopists at the 2nd Annual Midwest Advanced MINFLUX and Superresolution Workshop.
The workshop will be hosted online and in-person by the UGB Core at the University of Illinois at Urbana Champaign. The workshop will feature presentations and hands-on imaging sessions on an abberior MINFLUX and STEDYCON!
Registration is now open: abberior.co/MidwestWorkshop
#MINFLUX #superresolution #abberior
Upgraded MemBright Dyes fighting against phototoxicity with the evergreen COT moiety by @ZhixingChen2 - fantastic imaging ✨
The MemBright PM stains set a high bar for specific membrane labeling. But if you care about the phototoxicity under time-lapse and SR imaging?
Here we present a gentler palette -- PK Mem dyes. Tailored for live cell SIM/STED!
biorxiv.org/content/10.1101/…
Julian Kompa retweeted
We will again give an in-depth workshop on superresolution microscopy with #STED and #MINFLUX at EMBL Heidelberg in October. Register now!
#superresolution
🔬 Do you use super-resolution microscopy for your research?
If you want to learn more about the different methods of STED and MINFLUX nanoscopy, apply for our #EMBLSTED_MINFLUX course 📝
📅 21 – 26 October 2024
📍 EMBL Heidelberg
➡️ Apply by July 29
🔗 s.embl.org/eic24-01
