@amimspike

https://xitter.cf/t.co/kZgrziyU8p Visiting Assistant Professor @OberlinCollege 🧬 DNA replication, biochemistry, telomeres, naps 👩‍🔬 she/her

 Oberlin, OH
Joined November 2013
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63Media
And now officially published @NAR_Open 🎉 Now with some more experiments with Pol alpha...I'm looking forward to following up on these findings in the near future 😊 academic.oup.com/nar/advance…

Distinct RPA functions promote eukaryotic DNA replication initiation and elongation

Abstract. Replication protein A (RPA) binds single-stranded DNA (ssDNA) and serves critical functions in eukaryotic DNA replication, the DNA damage respons

academic.oup.com
Alexandra Pike@amimspike3 Oct 2022
In our recent preprint, we tested the requirements for the ssDNA-binding protein RPA in different stages of eukaryotic DNA replication… biorxiv.org/content/10.1101/…

Distinct RPA functions promote eukaryotic DNA replication initiation and elongation

Single-stranded DNA binding proteins (SSBs) are essential for DNA replication across all domains of life, but vary significantly in their structure and subunit composition. The eukaryotic SSB,...

biorxiv.org
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Alexandra Pike retweeted
Rajini Rao@madamscientist11 Feb 2025
BREAKING: Johns Hopkins along with 12 peer institutions and Assoc of Amer Univ sues to stop drastic cuts in NIH funding. This is separate from lawsuit brought by 22 states, which also includes Maryland. Hopkins prez cites our 600 clinical trials that would be crippled by the cut.
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Absolutely shocked that for an order containing 2 tubes of Gel Red and 1 RNaseA powder, @MilliporeSigma is charging us $61.69 in shipping plus $25 in ice. In what world???
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Our resource coordinator said we haven't been able to negotiate better shipping costs with Sigma. But there is no way this is reasonable, right?
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Also I cannot imagine that this order requires $25 of ice, considering that I literally have to boil the RNaseA before using it, and gel red is stored at room temp.
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Our Spotlight on beautiful work from the de Lange (@SarahWCai et al), Walz, and Cech labs. At the end, it is POT1 again: Phosphorylation allows human telomeric protein POT1 to recruit the C-rich strand end replication machinery: Molecular Cell cell.com/molecular-cell/full…
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I know yall biochemists have strong opinions about things....how do you feel about AKTA vs BioRad for FPLCs? Performance, user interface, maintenance, etc.
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Also considering I've only used AKTA before, but my department is looking to replace an end-of-life BioRad one (so misc parts/components are already here)
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Very excited that my meta-analysis of telomere length GWAS and experimental validation showing that POP5 and KBTBD6 are human telomere length regulation genes is out in @NatureComms! Shout out to my mentors @alexisjbattle and @RasikaMathias nature.com/articles/s41467-0…
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Plasmidsaurus reveals the spooky things lurking in your sequences...
In the first panel, a dinosaur reaches to remove the mask of what appears to the a tied-up ghost. In the second panel, the ghost is revealed to be a two-headed man labeled "concatemer."

ALT In the first panel, a dinosaur reaches to remove the mask of what appears to the a tied-up ghost. In the second panel, the ghost is revealed to be a two-headed man labeled "concatemer."

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Day 11/31 of #inktober2023 Prompt: WANDER Yellow Fluorescent Protein (Pdb: 1YFP). It lights up and lets us see how proteins wander through the cell. Next prompt is SPICEY. Please send suggestions. @PDBeurope @buildmodels @inktober #sciart
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Alexandra Pike retweeted
The Nobel Prize
@NobelPrize2 Oct 2023
The discoveries by Katalin Karikó and Drew Weissman – awarded the 2023 #NobelPrize in Physiology or Medicine – were critical for developing effective mRNA vaccines against COVID-19 during the pandemic that began in early 2020. Through their groundbreaking findings, this year’s laureates have fundamentally changed our understanding of how mRNA interacts with our immune system. They have contributed to the unprecedented rate of vaccine development during one of the greatest threats to human health in modern times.
A blue background with COVID-19 virus and a yellow strand of modified mRNA. Also shown is the chemical structure of pseudouridine, an RNA base that was important in the prize-awarded discovery. The graphic represents the 2023 Nobel Prize in Physiology or Medicine awarded to Katalin Karinkó and Drew Weissman who received the Nobel Prize in Physiology or Medicine for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19.

ALT A blue background with COVID-19 virus and a yellow strand of modified mRNA. Also shown is the chemical structure of pseudouridine, an RNA base that was important in the prize-awarded discovery. The graphic represents the 2023 Nobel Prize in Physiology or Medicine awarded to Katalin Karinkó and Drew Weissman who received the Nobel Prize in Physiology or Medicine for their discoveries concerning nucleoside base modifications that enabled the development of effective mRNA vaccines against COVID-19.

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Chaotic evil: someone left a permanent marker on the whiteboard tray in the classroom I was using this morning
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When it's the first day of class AND you get a "Manuscript Accepted" email 🎉 SO MANY FEELS
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What a pleasant surprise! PDBe featured our recently published structure of human POT1 bound to the telomeric ds-ss junction. See 👇 thread for more details of our study. xitter.cf/LabNandakumar/status/1…
Protein Data Bank@PDBeurope30 Aug 2023
This week's #PDB release has 315 new entries (PDBe.org/latest)! Here is a structure of ‘Protection of telomeres 1’ / POT1 protein bound to ds-DNA with an overhang. ‘POT-hole’ - see orange-red phosphate at end of shorter DNA. Published @Science ➡️ PDBe.org/8sh1
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Our biggest discovery yet (IMO). The 5’ ends of our chromosomes are capped(!) by #telomeric protein POT1 to prevent their misrecognition as broken DNA. Brilliant work of Drs. Valerie Tesmer and Kirsten Brenner @ScienceMagazine science.org/doi/10.1126/scie… 1/n

Human POT1 protects the telomeric ds-ss DNA junction by capping the 5′ end of the chromosome

POT1 binds the 5ʹ end of the telomeric double-strand–single-strand DNA junction to prevent human chromosome ends from being sensed as DNA damage.

science.org
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DEADLIEST fires in modern US history I’m hearing people of Maui finding bodies in cars, houses, streets, and in the ocean The continental media MUST do more to capture the stories of the Native Hawaiian and local communities of Lāhainā and Maui washingtonpost.com/climate-e…

How the Maui fires compare with some of the deadliest U.S. wildfires

The Maui wildfire outbreak is one of the deadliest in U.S. history.

washingtonpost.com
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🙌🏽PLEASE SHARE Our Native Hawaiian communities should not be silenced in our ancestral lands. Unreal. Mahalo @aku_maka Paele_Kulani. Let’s keep amplifying #indigenousVoices – at Mountain View, CA
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When you go back to your postdoc lab to finish up some experiments but then the piece of equipment you wanted to use stops working 🙃😵‍💫
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