Michal Holčapek (@holcapek) profile picture

Michal Holčapek

@holcapek

Research interests: lipidomic analysis, UHPLC/MS, UHPSFC/MS, mass spectrometry, cancer biomarkers

 University of Pardubice
holcapek.upce.cz/
Joined December 2013
  • Tweets71
  • Following238
  • Followers367
Thank you for citing #tidyplots 🙏 Jakub Idkowiak et al. Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data. Nature Communications (2025). doi.org/10.1038/s41467-025-6… #rstats #dataviz #phd

Best practices and tools in R and Python for statistical processing and visualization of lipidomics...

Nature Communications - Mass spectrometry-based lipidomics and metabolomics generate large, complex datasets requiring effective analysis. Here, authors review key statistical and visualization...

nature.com
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Michal Holčapek retweeted
Kermit Murray (@kermitmurray) profile picture
Kermit Murray@kermitmurrayMay 28
A lipidomics roadmap: from basic research to societal challenges dlvr.it/TSmSzk (RSS) #nature #MassSpecRSS
Image from Kermit Murray (@kermitmurray)
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Lesley Goodburn (@lgoodbu) profile picture
Lesley Goodburn@lgoodbuMar 2
Pilot study of screening method for pancreatic cancer using lipidomic profiling of plasma or serum | Communications Medicine nature.com/articles/s43856-0…

Pilot study of screening method for pancreatic cancer using lipidomic profiling of plasma or serum

Communications Medicine - Peterka et al. develop a noninvasive blood-based lipidomic test that distinguishes pancreatic cancer patients from healthy controls. The test achieves >95%...

nature.com
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Holčapkovic (@holcapkovi54219) profile picture
Holčapkovic@holcapkovi5421924 Nov 2025
🌟 We are thrilled to announce that another article has just been published in @an_chem Our study, presents a robust and high-throughput workflow for quantifying less polar lipid species in complex biological samples. 🧪✨ doi.org/10.1021/acs.analchem… #lipidomics #massspectrometry

Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reverse...

Abstract. The quantitative analysis of lipids is challenging due to their structural diversity and the coexistence of numerous isomers in biological sample

pubs.acs.org
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Holčapkovic (@holcapkovi54219) profile picture
Holčapkovic@holcapkovi5421919 Nov 2025
🚀 New paper out! Our study “Enhancing metabolite coverage using dedicated individual polarity modes in HILIC-MS” is now published in ABC!🙌 link.springer.com/article/10…

Enhancing metabolite coverage using dedicated mobile phases for individual polarity modes in...

Analytical and Bioanalytical Chemistry - We have developed a new hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) method with mobile phases optimized for high metabolite...

link.springer.com
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3/3📘 Published by the International Lipidomics Society in Science Signaling (May 20, 2025). 👉 DOI: 10.1126/scisignal.adw1245 #health #massspectrometry #UPCE
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2/3🔬 The recommendations aim to improve reproducibility in LC-MS/MS analysis, a technique that detects 200+ lipid species in one run. Oxylipins also help explain how common drugs like aspirin work. #research #science
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1/3🧪 New guidelines for analyzing oxylipins – fatty molecules linked to inflammation, cancer & dementia – were developed by nearly 100 scientists from 70+ institutions worldwide. These lipids are crucial for health and notoriously hard to measure. #lipidomics #UPCE
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Lenka Dub (@DubLenka10222) profile picture
Lenka Dub@DubLenka1022229 Mar 2025
Novel Charge-Switch Derivatization Method Using 3-(Chlorosulfonyl)benzoic Acid for Sensitive RP-UHPLC/MS/MS Analysis of Acylglycerols, Sterols, and Prenols | Analytical Chemistry pubs.acs.org/doi/10.1021/acs…
Novel Charge-Switch Derivatization Method Using 3‑(Chlorosulfonyl)benzoic Acid for Sensitive...

Novel Charge-Switch Derivatization Method Using 3‑(Chlorosulfonyl)benzoic Acid for Sensitive...

Abstract. Chemical derivatization involves the reaction of an analyte with a derivatization agent to modify its structure, improving the peak shape, chroma

pubs.acs.org
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Our new article in Analytical Chemistry unveils breakthrough 4D UHPLCxUHPSFC/MS/MS method for comprehensive detection of lipids: pubs.acs.org/doi/10.1021/acs…

Four-Dimensional Lipidomic Analysis Using Comprehensive Online UHPLC × UHPSFC/Tandem Mass Spectro...

Abstract. Multidimensional chromatography offers enhanced chromatographic resolution and peak capacity, which are crucial for analyzing complex samples. Th

pubs.acs.org
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Aktivuje prospěšný receptor CAR a blokuje škodlivé účinky PXR. Studie na myších ukázala skvělé výsledky a objev publikoval prestižní časopis Nature Communications. (3/3) nature.com/articles/s41467-0…
The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

Nature Communications - CAR and PXR receptors are known to regulate metabolism, however, there is no dual human ligand suitable for therapy. Here, the authors show a CAR agonist/PXR antagonist,...

nature.com
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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards pubmed.ncbi.nlm.nih.gov/3936…
Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials...

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials...

In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four...

pubmed.ncbi.nlm.nih.gov
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Our cooperation with Petr Pávek group resulted in Nat Commun paper: The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced hypercholesterolemia model nature.com/articles/s41467-0…
The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

Nature Communications - CAR and PXR receptors are known to regulate metabolism, however, there is no dual human ligand suitable for therapy. Here, the authors show a CAR agonist/PXR antagonist,...

nature.com
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New article on our software is available online Lipid Quant 2.1: open-source software for identification and quantification of lipids measured by lipid class separation QTOF high-resolution mass spectrometry methods: doi.org/10.1016/j.chemolab.2…
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Our review article "Recent Advances, Challenges, and Future Directions in the Mass Spectrometry Analysis of Glycosphingolipids in Biological Samples" is now available online: doi.org/10.1016/j.trac.2024.…
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Our new article "HILIC/MS quantitation of low-abundant phospholipids and sphingolipids in human plasma and serum: Dysregulation in pancreatic cancer" is just published: sciencedirect.com/science/ar…
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Our new paper is on complex characterization of GSL is published: doi.org/10.1016/j.jbc.2023.1…
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Michal Holčapek retweeted
IMSC2022 (@imsc2022) profile picture
IMSC2022@imsc202231 Aug 2022
The third award of today: the first Jochen Franzen award: Shane Ellis @EllisMSILab @MolHorizons @UOW Congratulations! @BrukerMassSpec @bruker @anzsms @denvms @TheIMSF
Image from IMSC2022 (@imsc2022)
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Michal Holčapek retweeted
Kermit Murray (@kermitmurray) profile picture
Kermit Murray@kermitmurray8 Aug 2022
Introducing the Lipidomics Minimal Reporting Checklist dlvr.it/SWDjl3 #nature #MassSpecPub
Image from Kermit Murray (@kermitmurray)
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Online now! Introducing the Lipidomics Minimal Reporting Checklist dlvr.it/SWDjDN
Image from Nature Metabolism (@NatMetabolism)
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