Michal Holčapek (@holcapek) profile picture

Michal Holčapek

@holcapek

Research interests: lipidomic analysis, UHPLC/MS, UHPSFC/MS, mass spectrometry, cancer biomarkers

 University of Pardubice
holcapek.upce.cz/
Joined December 2013
  • Tweets71
  • Following238
  • Followers367
Thank you for citing #tidyplots 🙏 Jakub Idkowiak et al. Best practices and tools in R and Python for statistical processing and visualization of lipidomics and metabolomics data. Nature Communications (2025). doi.org/10.1038/s41467-025-6… #rstats #dataviz #phd

Best practices and tools in R and Python for statistical processing and visualization of lipidomics...

Nature Communications - Mass spectrometry-based lipidomics and metabolomics generate large, complex datasets requiring effective analysis. Here, authors review key statistical and visualization...

nature.com
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Michal Holčapek retweeted
Kermit Murray (@kermitmurray) profile picture
Kermit Murray@kermitmurrayMay 28
A lipidomics roadmap: from basic research to societal challenges dlvr.it/TSmSzk (RSS) #nature #MassSpecRSS
Image from Kermit Murray (@kermitmurray)
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Lesley Goodburn (@lgoodbu) profile picture
Lesley Goodburn@lgoodbuMar 2
Pilot study of screening method for pancreatic cancer using lipidomic profiling of plasma or serum | Communications Medicine nature.com/articles/s43856-0…

Pilot study of screening method for pancreatic cancer using lipidomic profiling of plasma or serum

Communications Medicine - Peterka et al. develop a noninvasive blood-based lipidomic test that distinguishes pancreatic cancer patients from healthy controls. The test achieves >95%...

nature.com
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Michal Holčapek retweeted
Holčapkovic (@holcapkovi54219) profile picture
Holčapkovic@holcapkovi5421924 Nov 2025
🌟 We are thrilled to announce that another article has just been published in @an_chem Our study, presents a robust and high-throughput workflow for quantifying less polar lipid species in complex biological samples. 🧪✨ doi.org/10.1021/acs.analchem… #lipidomics #massspectrometry

Comprehensive Quantitative Profiling of Less Polar Lipids in Human Plasma Using Validated Reverse...

Abstract. The quantitative analysis of lipids is challenging due to their structural diversity and the coexistence of numerous isomers in biological sample

pubs.acs.org
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Holčapkovic (@holcapkovi54219) profile picture
Holčapkovic@holcapkovi5421919 Nov 2025
🚀 New paper out! Our study “Enhancing metabolite coverage using dedicated individual polarity modes in HILIC-MS” is now published in ABC!🙌 link.springer.com/article/10…

Enhancing metabolite coverage using dedicated mobile phases for individual polarity modes in...

Analytical and Bioanalytical Chemistry - We have developed a new hydrophilic interaction liquid chromatography-mass spectrometry (HILIC-MS) method with mobile phases optimized for high metabolite...

link.springer.com
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3/3📘 Published by the International Lipidomics Society in Science Signaling (May 20, 2025). 👉 DOI: 10.1126/scisignal.adw1245 #health #massspectrometry #UPCE
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2/3🔬 The recommendations aim to improve reproducibility in LC-MS/MS analysis, a technique that detects 200+ lipid species in one run. Oxylipins also help explain how common drugs like aspirin work. #research #science
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1/3🧪 New guidelines for analyzing oxylipins – fatty molecules linked to inflammation, cancer & dementia – were developed by nearly 100 scientists from 70+ institutions worldwide. These lipids are crucial for health and notoriously hard to measure. #lipidomics #UPCE
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Michal Holčapek retweeted
Lenka Dub (@DubLenka10222) profile picture
Lenka Dub@DubLenka1022229 Mar 2025
Novel Charge-Switch Derivatization Method Using 3-(Chlorosulfonyl)benzoic Acid for Sensitive RP-UHPLC/MS/MS Analysis of Acylglycerols, Sterols, and Prenols | Analytical Chemistry pubs.acs.org/doi/10.1021/acs…
Novel Charge-Switch Derivatization Method Using 3‑(Chlorosulfonyl)benzoic Acid for Sensitive...

Novel Charge-Switch Derivatization Method Using 3‑(Chlorosulfonyl)benzoic Acid for Sensitive...

Abstract. Chemical derivatization involves the reaction of an analyte with a derivatization agent to modify its structure, improving the peak shape, chroma

pubs.acs.org
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Aktivuje prospěšný receptor CAR a blokuje škodlivé účinky PXR. Studie na myších ukázala skvělé výsledky a objev publikoval prestižní časopis Nature Communications. (3/3) nature.com/articles/s41467-0…
The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

The hypolipidemic effect of MI-883, the combined CAR agonist/ PXR antagonist, in diet-induced...

Nature Communications - CAR and PXR receptors are known to regulate metabolism, however, there is no dual human ligand suitable for therapy. Here, the authors show a CAR agonist/PXR antagonist,...

nature.com
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Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials via authentic standards pubmed.ncbi.nlm.nih.gov/3936…
Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials...

Concordant inter-laboratory derived concentrations of ceramides in human plasma reference materials...

In this community effort, we compare measurements between 34 laboratories from 19 countries, utilizing mixtures of labelled authentic synthetic standards, to quantify by mass spectrometry four...

pubmed.ncbi.nlm.nih.gov
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Michal Holčapek retweeted
IMSC2022 (@imsc2022) profile picture
IMSC2022@imsc202231 Aug 2022
The third award of today: the first Jochen Franzen award: Shane Ellis @EllisMSILab @MolHorizons @UOW Congratulations! @BrukerMassSpec @bruker @anzsms @denvms @TheIMSF
Image from IMSC2022 (@imsc2022)
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Michal Holčapek retweeted
Kermit Murray (@kermitmurray) profile picture
Kermit Murray@kermitmurray8 Aug 2022
Introducing the Lipidomics Minimal Reporting Checklist dlvr.it/SWDjl3 #nature #MassSpecPub
Image from Kermit Murray (@kermitmurray)
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Online now! Introducing the Lipidomics Minimal Reporting Checklist dlvr.it/SWDjDN
Image from Nature Metabolism (@NatMetabolism)
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Michal Holčapek retweeted
Denise Wolrab (@DeniseWolrab) profile picture
Denise Wolrab@DeniseWolrab2 Feb 2022
We are very honored that we got awarded by the University of Pardubice for our lipidomic profiling study to detect pancreatic cancer published in @NatureComms. Thank you @UniPardubice!!! @holcapek @RJirasko @IdkowiakJakub
Image from Denise Wolrab (@DeniseWolrab)
Image from Denise Wolrab (@DeniseWolrab)
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Retention dependences support identification of #lipid species in human #plasma by reversed-phase #UHPLC/MS by Michal Holčapek et al @UniPardubice @holcapek @DeniseWolrab @ZuzanaVanko @RJirasko #lipidomics #ABCcountdown 🎉 #ABC20thAnnivIssue link.springer.com/article/10…
Image from Anal Bioanal Chem (@AnalBioanalChem)
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Michal Holčapek retweeted
Denise Wolrab (@DeniseWolrab) profile picture
Denise Wolrab@DeniseWolrab10 Jan 2022
Our study for the differentiation of serum lipidomic profiles of PDAC and control samples is online. I´m very excited and proud to share "my scientific baby". @holcapek @UniPardubice @NatureComms #lipidomics #pancreaticcancer disq.us/t/4499r8m
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Michal Holčapek retweeted
Denise Wolrab (@DeniseWolrab) profile picture
Denise Wolrab@DeniseWolrab10 Jan 2022
I would like to thank all collaborators!!! @LipidomicsRgb @SlingLipidomics @FriedeckyGroup @Ahrends15 and all the others :)
Denise Wolrab (@DeniseWolrab) profile pictureDenise Wolrab@DeniseWolrab10 Jan 2022
Our study for the differentiation of serum lipidomic profiles of PDAC and control samples is online. I´m very excited and proud to share "my scientific baby". @holcapek @UniPardubice @NatureComms #lipidomics #pancreaticcancer disq.us/t/4499r8m
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Michal Holčapek retweeted
Denise Wolrab (@DeniseWolrab) profile picture
Denise Wolrab@DeniseWolrab17 Dec 2021
Our data processing solution for lipid class separation approaches hyphenated to MS is online now :) @holcapek @FemalesInMS @bioinformatics LipidQuant 1.0: automated data processing in lipid class separation–mass spectrometry quantitative workflows academic.oup.com/bioinformat…
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Michal Holčapek retweeted
Tomas Cajka (@TomasCajka) profile picture
Tomas Cajka@TomasCajka12 Oct 2021
Happy to share our recent paper published in @CellReports on metabolomics atlas of oral 13C-glucose tolerance test in mice! #GTTAtlas #LIMeX #metabolomics #lipidomics #fluxomics cell.com/cell-reports/fullte…
Image from Tomas Cajka (@TomasCajka)
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