Structural biologist in pharma. Post doc @GonenLab @UCLA. PhD @theJensenLab @Caltech. https://xitter.cf/t.co/KqIWKvaWLd
Tovaangar
Joined April 2014
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Sara J. Weaver, Ph.D. retweeted
APEX peroxidase is a versatile tool for proximity labeling and electron microscopy (EM) but its requirement for toxic H2O2 is a liability. We now report LaccID, a multicopper oxidase evolved from an ancestral fungal laccase that oxidizes aromatic substrates using non-toxic O2 instead of H2O2. We use LaccID for EM imaging of cultured cells and fly brains, and for proteomic mapping of the changing surface composition of T cells that engage with tumor cells via antigen-specific T cell receptors.
Sara J. Weaver, Ph.D. retweeted
Glad to see this online in @PNASNews (pubmed.ncbi.nlm.nih.gov/3935…). I started this project many moons ago at Caltech. While trying to dissect the Legionella Type 4 Secretion System (T4SS), I found deletion of two T4SS components (dotI/dotJ) abrogates ATPase recruitment.
Sara J. Weaver, Ph.D. retweeted
Vogel lab (WashU) had similar conclusions from their biochemical analysis. We decided to dig deep! Sukhi spent nearly 3 years doing meticulous biochemical and genetic analysis to show that DotJ, DotI, and IcmT are receptors for the T4SS’ unique 3-component ATPase complex.
Sara J. Weaver, Ph.D. retweeted
hey #teamtomo if you are looking to add to your toolkit for molecular identification inside cells, our preprint is now out!
ExoSloNano: Multi-Modal Nanogold Tags for identi-fication of Macromolecules in Live Cells & Cryo-Electron Tomograms biorxiv.org/cgi/content/shor… #biorxiv_biophys
Sara J. Weaver, Ph.D. retweeted
Filomeno Sánchez Rodríguez et al.: Using deep-learning predictions reveals a large number of register errors in PDB depositions #DeepLearning #ProteinDataBank #StructureValidation @livuni @uniofyork... #IUCr journals.iucr.org/paper?S205…
Sara J. Weaver, Ph.D. retweeted
👀❄️🔬Check it out from Jae Yang, Josh Mitchell, Craig Bingman, Deane Mosher, & me!! Tour de force of #cryoEM, #microED, #cryoET, & #cryoFIB to solve the in situ crystalline structure of the #Eosinophil major basic protein-1! biorxiv.org/cgi/content/shor… Congrats! @UWBiochem
Sara J. Weaver, Ph.D. retweeted
Yesterday a Nobel prize was given for alpha fold. Alpha fold could not exist without the Protein Data Bank (PDB), a public database funded by US taxpayers. Every structural biologist in the world that deposited their structure to the PDB indirectly helped train alpha fold.
Sara J. Weaver, Ph.D. retweeted
It's happening! We're excited to be hosting ~300 attendees for the 9th SoCal CryoEM Symposium @USC tomorrow. @USCDornsife @USCBridge dornsife.usc.edu/bridge-inst…
Sara J. Weaver, Ph.D. retweeted
New preprint out from the MonteRosa gang on computational approaches to mine the CRBN target space. Revealed new neosubstrates recognition modes including helical motifs and molecular surface mimicry. Lots to still learn about redirecting CRBN!
biorxiv.org/content/10.1101/…
I would like snack on a bottle of #sigmaaldrichchocolate to fuel my science. Thanks @Merck_lifesci
Sara J. Weaver, Ph.D. retweeted
"Ever wondered how α-latrotoxin, the black widow spider's 🕷️ neurotoxin, turns into a cation-selective channel? Check out our latest manuscript! rdcu.be/dWez3 @NatureComms #Neuroscience #Toxin #cryoEM @Sfb1348 @uni_muenster
Sara J. Weaver, Ph.D. retweeted
Maymay at her award seminar this morning. Many congratulations!! Very well deserved!
Sara J. Weaver, Ph.D. retweeted
This is damning. Another cryo-EM retraction and a paper based on zero data onlinelibrary.wiley.com/doi/…
Sara J. Weaver, Ph.D. retweeted
CS vs RelionCor/CtfFind4 on the same 100 movies. Tried pretty much every setting in RelionCor/CtfFind4 but cannot get the CTF to be estimated well. The movies look good by eye and Thon rings are visible. Any ideas what to try if I was commited to use Relion/CTFfind?
Sara J. Weaver, Ph.D. retweeted
Dear cryo-EM women scientists,
We want to honor you and your contributions at BPS 2025.
This year, the Cryo-EM subgroup will honor a mid/senior-career woman scientist as the inaugural June Almeida awardee.
June Almeida is the virologist who first discovered the coronavirus in 1967 using negative stain electron microscopy. Her story, like many women we know, is remarkable. womenshistory.org/education-…
It is in the spirit of June Almeida’s innovative research that this award is named. The awardee, in addition to receiving an honorarium, will present their research at the BPS CryoEM Subgroup Symposium. The deadline for nominations for the Almeida Award is November 1st, and details of the award and the application process can be found at (biophysics.org/Awards-Fundin…). Nomination packets should be sent to [email protected].
Questions? Contact @OliBClarke, me, @MeghnaG27 @PilarCossio2 or @10feng
♥️We sincerely hope to see you at BPS 2025 in LA to celebrate achievements and advances in cryo-EM!
Sara J. Weaver, Ph.D. retweeted
We are thrilled to welcome the incomparable Yiorgo Skiniotis and his lab!
Yiorgo will also lead the new Center of Excellence for Structural Cell Biology, to advance cryoET and other cutting-edge approaches @StJudeResearch, paving the way for new, exciting biological insights!
Sara J. Weaver, Ph.D. retweeted
Our article on the superworm pandemic is out in the Oct 3 print edition of Cell. Do any of my colleagues here subscribe to the print edition, and would you be willing to give me your copy when you're done with it? I'd love to keep a physical version.
Sara J. Weaver, Ph.D. retweeted
Our paper CryoBench: Diverse & challenging datasets for the heterogeneity problem in cryo-EM has been accepted at #NeurIPS2024 as a #spotlight! 🎉
🔗 Project: cryobench.cs.princeton.edu
📄 Paper: arxiv.org/pdf/2408.05526
💻 Code: github.com/ml-struct-bio/Cry…
Congrats to all authors!
Sara J. Weaver, Ph.D. retweeted
Excited and deeply honored to receive the inaugural @BiophysicalSoc Early Independent Career Award!!
So grateful for this recognition, my collaborators, mentors, and most importantly, the @BarnesLab1 team who make it all happen!! This award is truly ours!!! 🙌🏾🫶🏾🍾
Christopher O. Barnes to Receive 2025 Early Independent Career Award ow.ly/X9Ft50TuvW6
@cobarnes27 @Stanford
