@schmidburgk

Group leader @UniklinikBonn @UniBonn

Joined October 2011
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Can AI-designed binders degrade, aggregate, stain and enrich endogenous proteins in human cells? Check out our new preprint! biorxiv.org/content/10.1101/…

Transposon-Display of AI-designed binders enables manipulation of the proteome in human cells

Transposon-Display is a highly scalable screening method that links proteins to their encoding DNA during expression in E. coli via a mutant transposase. Leveraging this system, we identified...

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We are grateful to the Hans und Ria Messer Stiftung and the cluster of excellence @ImmunoSens for supporting our research at @UniBonn @UniklinikBonn
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Please also check out another exciting work on AI-based protein design, which was a fantastic team effort with Michael Hölzel, Gregor Hagelueken, Tobias Bald and many colleagues at @UniklinikBonn biorxiv.org/content/10.1101/…

Development of AI-designed protein binders for detection and targeting of cancer cell surface...

Artificial intelligence (AI)-based protein design opens new avenues for the rapid generation of new research tools and therapeutics, but experimental validation lags behind the computational design...

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How could we identify key genes involved in any biological process and cell type? Please read on about Nuclear In-Situ Sequencing (NIS-Seq) nature.com/articles/s41587-0…

NIS-Seq enables cell-type-agnostic optical perturbation screening

Nature Biotechnology - An optical pooled screening method can correlate phenotypes and perturbations in any nucleated cell type.

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6| Thanks to the entire team including @p_knp_33, @AlexH_UB, @blumenstock_k, and the labs of @FISchmidtLab, @eickelatz, @Doc_Bald, @RaykBehrendt, and @appsilon. Thanks to @ImmunoSens @UniBonn @UniklinikBonn for supporting our work.
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7| Please also see very exciting complementary approaches by the @eric_lubeck, Aviv Regev and @GaublommeL labs, improving optical perturbation screening using different tricks. nature.com/articles/s41587-0… nature.com/articles/s41587-0….
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Jonathan Schmid-Burgk retweeted
Nature Biotechnology
@NatureBiotech19 Dec 2024
NIS-Seq enables cell-type-agnostic optical perturbation screening go.nature.com/3P35toD
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Jonathan Schmid-Burgk retweeted
Max Wilkinson@maxewilkinson29 Aug 2024
My favourite discovery ever has just come online. Can I please tell you about some seriously wacky molecular biology? The story starts with a reverse transcriptase that SOMEHOW defends bacteria from viruses. (👇 I recommend sound ON for the video 🎹) 1/
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1/8 How can we enrich cells with a defined transcriptional state, but without killing them? Please read on! biorxiv.org/content/10.1101/…

Sensing endogenous RNA in living human cells using a CRISPR-activated protease

Most techniques used to detect specific mRNAs in eukaryotic cells require to extract nucleic acids and thereby kill the cells. A programmable sensor for monitoring endogenous transcripts in living...

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7/8 This project was inspired by previous work by the Hagelueken lab (@gha14, @NielsSchneberg1) and @ChristopheRoui9, characterizing the CRISPR-associated Lon protease CalpL nature.com/articles/s41586-0….

Antiviral signalling by a cyclic nucleotide activated CRISPR protease

Nature - A CalpL–CalpT–CalpS cascade mediated by cyclic oligoadenylates is identified as a mechanism to detect viral RNA and activate subsequent antivirus responses in microorganisms.

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8/8 Also please check out a fascinating related work by the @doudna_lab biorxiv.org/content/10.1101/…

Single-molecule live-cell RNA imaging with CRISPR-Csm

High-resolution, real-time imaging of RNA is essential for understanding the diverse, dynamic behaviors of individual RNA molecules in single cells. However, single-molecule live-cell imaging of...

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