
PhD graduate at CINVESTAV Unidad Irapuato (@CinvestavIra 🇲🇽). Algal biotechnology #Botryococcus and 🌱 #PlantTOR signaling pathway. @tkozawa.bsky.social
- Tweets2.2K
- Following1K
- Followers631
- Likes6.1K

ALT Fig. 4. Glucose-TOR signaling drives callose deposition at plasmodesmata (PD) through the PP2A-ABI5- PDCB signaling axis. (A and B) TOR and PP2A antagonistically regulate callose deposition at PD in leaves. Callose deposits in cell walls were stained with aniline blue and visualized using confocal fluorescence microscopy. Inhibiting TOR with Torin2 (T2) significantly decreased PD callose (n ≥ 27, P < 10−2), whereas inhibiting PP2A with either CAN or OA significantly increased PD callose (n ≥ 27, P < 10−7). Representative images shown in (B). (C and D) Overexpressing the transcription factor ABI5 is sufficient to significantly increase callose deposition at PD compared to mock controls (35SPRO:GFP) (n ≥ 27, P < 10−7). (E and F) Silencing ABI5 is sufficient to reduce callose deposition at PD compared to mock controls (n ≥ 16, P < 10−1). Oppositely, silencing DSE1 increases PD callose, as expected (n ≥ 27, P < 10−7). (G) Glucose-TOR-ABI5 signaling induces PDCB1 and PDCB2 expression...

































