ALT Mary Ippolito and I posing in front of a mural in San Francisco.
ALT My parents, Janine and Gerald Goertsen, and I, in front of art in the Chen Institute for Neuroscience at Caltech.
ALT Gradinaru Lab at the Athenaeum in 2022.
ALT Ph.D. defense title slide: "Expanding adeno-associated viral capsid engineering to multiple variable regions for diversified tropism"
ALT On the left, co-localization of transgene expression (in green) and ATII cells (in magenta, stained with anti-proSPC) within the lung is indicated with arrowheads. On the right, quantification of ATII cell transduction is displayed, where AAV9.452sub.LUNG1 is ~30-fold stronger than AAV9.
ALT In the top panel, the enrichment values for all sequences obtained after the second round of selection are displayed. Both AAV9 and discovered variant AAV9.452sub.LUNG1 are indicated. Enrichment values are colored by animal. On the bottom left, transgene expression (green) in the lung is compared between AAV9 and AAV9.452sub.LUNG1. On the bottom right, GFP+ nuclei per square millimeter lung tissue area is plotted, where AAV9.452sub.LUNG1 is ~18-fold better than AAV9.
ALT On the left, the AAV9 capsid structure is shown, colored by the distance from the center. On the right, the surface exposed variable regions of AAV9 are displayed, with the furthest protruding amino acids (AA452-458) highlighted. The highlighted residues are the site of engineering for this research.
